Therapeutic genome editing: prospects and challenges
Recent advances in the development of genome editing technologies based on
programmable nucleases have substantially improved our ability to make precise changes …
programmable nucleases have substantially improved our ability to make precise changes …
Genome editing: the road of CRISPR/Cas9 from bench to clinic
A Eid, MM Mahfouz - Experimental & Molecular Medicine, 2016 - nature.com
Molecular scissors engineered for site-specific modification of the genome hold great
promise for effective functional analyses of genes, genomes and epigenomes and could …
promise for effective functional analyses of genes, genomes and epigenomes and could …
Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements
CRISPR–Cas9 is poised to become the gene editing tool of choice in clinical contexts. Thus
far, exploration of Cas9-induced genetic alterations has been limited to the immediate …
far, exploration of Cas9-induced genetic alterations has been limited to the immediate …
DNA-free genetically edited grapevine and apple protoplast using CRISPR/Cas9 ribonucleoproteins
The combined availability of whole genome sequences and genome editing tools is set to
revolutionize the field of fruit biotechnology by enabling the introduction of targeted genetic …
revolutionize the field of fruit biotechnology by enabling the introduction of targeted genetic …
DNA repair profiling reveals nonrandom outcomes at Cas9-mediated breaks
M van Overbeek, D Capurso, MM Carter… - Molecular cell, 2016 - cell.com
The repair outcomes at site-specific DNA double-strand breaks (DSBs) generated by the
RNA-guided DNA endonuclease Cas9 determine how gene function is altered. Despite the …
RNA-guided DNA endonuclease Cas9 determine how gene function is altered. Despite the …
Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
Binding specificity of Cas9–guide RNA complexes to DNA is important for genome-
engineering applications; however, how mismatches influence target recognition/rejection …
engineering applications; however, how mismatches influence target recognition/rejection …
CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites
R Jayavaradhan, DM Pillis, M Goodman… - Nature …, 2019 - nature.com
Precise genome editing/correction of DNA double-strand breaks (DSBs) induced by
CRISPR-Cas9 by homology-dependent repair (HDR) is limited by the competing error-prone …
CRISPR-Cas9 by homology-dependent repair (HDR) is limited by the competing error-prone …
Microhomologies are prevalent at Cas9-induced larger deletions
DDG Owens, A Caulder, V Frontera… - Nucleic acids …, 2019 - academic.oup.com
The CRISPR system is widely used in genome editing for biomedical research. Here, using
either dual paired Cas9D10A nickases or paired Cas9 nuclease we characterize …
either dual paired Cas9D10A nickases or paired Cas9 nuclease we characterize …
Cas9-induced large deletions and small indels are controlled in a convergent fashion
Repair of Cas9-induced double-stranded breaks results primarily in formation of small
insertions and deletions (indels), but can also cause potentially harmful large deletions …
insertions and deletions (indels), but can also cause potentially harmful large deletions …
An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells
Human pluripotent stem cells (hPSCs) represent a unique opportunity for understanding the
molecular mechanisms underlying complex traits and diseases. CRISPR/Cas9 is a powerful …
molecular mechanisms underlying complex traits and diseases. CRISPR/Cas9 is a powerful …