Recent advances in droplet microfluidics for single-cell analysis

Z Jiang, H Shi, X Tang, J Qin - TrAC Trends in Analytical Chemistry, 2023 - Elsevier
Single-cell manipulation and analysis is essential for studying many fundamental biological
processes and discovering cellular heterogeneity. Multi-parameter studies of intercellular …

Advanced microfluidic technologies for lipid nano-microsystems from synthesis to biological application

BG Carvalho, BT Ceccato, M Michelon, SW Han… - Pharmaceutics, 2022 - mdpi.com
Microfluidics is an emerging technology that can be employed as a powerful tool for
designing lipid nano-microsized structures for biological applications. Those lipid structures …

Microfluidic and nanofluidic intracellular delivery

J Hur, AJ Chung - Advanced Science, 2021 - Wiley Online Library
Innate cell function can be artificially engineered and reprogrammed by introducing
biomolecules, such as DNAs, RNAs, plasmid DNAs, proteins, or nanomaterials, into the …

A comprehensive review on intracellular delivery

D Morshedi Rad, M Alsadat Rad… - Advanced …, 2021 - Wiley Online Library
Intracellular delivery is considered an indispensable process for various studies, ranging
from medical applications (cell‐based therapy) to fundamental (genome‐editing) and …

High-efficiency quantitative control of mitochondrial transfer based on droplet microfluidics and its application on muscle regeneration

J Sun, HTJ Lo, L Fan, TL Yiu, A Shakoor, G Li… - Science …, 2022 - science.org
Mitochondrial transfer is a spontaneous process to restore damaged cells in various
pathological conditions. The transfer of mitochondria to cell therapy products before their …

Highly efficient transfection of human primary T lymphocytes using droplet-enabled mechanoporation

B Joo, J Hur, GB Kim, SG Yun, AJ Chung - ACS nano, 2021 - ACS Publications
Whole-cell-based therapy has been extensively used as an effective disease treatment
approach, and it has rapidly changed the therapeutic paradigm. To fully accommodate this …

Liposomal delivery of CRISPR/Cas9

S Zhen, X Li - Cancer gene therapy, 2020 - nature.com
Liposomes are one of the most widely investigated carriers for CRISPR/Cas9 delivery. The
surface properties of liposomal carriers, including the surface charge, PEGylation, and …

Recent Advances in Microfluidic Platforms for Programming Cell‐Based Living Materials

P Zhang, N Shao, L Qin - Advanced Materials, 2021 - Wiley Online Library
Cell‐based living materials, including single cells, cell‐laden fibers, cell sheets, organoids,
and organs, have attracted intensive interests owing to their widespread applications in …

Recent advances in droplet-based microfluidic technologies for biochemistry and molecular biology

J Sánchez Barea, J Lee, DK Kang - Micromachines, 2019 - mdpi.com
Recently, droplet-based microfluidic systems have been widely used in various biochemical
and molecular biological assays. Since this platform technique allows manipulation of large …

Droplet manipulation on bioinspired slippery surfaces: From design principle to biomedical applications

X Wang, Z Zhuang, X Li, X Yao - Small Methods, 2024 - Wiley Online Library
Droplet manipulation with high efficiency, high flexibility, and programmability, is essential
for various applications in biomedical sciences and engineering. Bioinspired liquid‐infused …