Mechanistic and structural complexity in the site-specific recombination pathways of Int and FLP
A Landy - Current opinion in genetics & development, 1993 - Elsevier
This review focuses on two of the approximately 30 members of the diverse Int family of site-
specific recombinases. The λ recombination system represents those reactions involving …
specific recombinases. The λ recombination system represents those reactions involving …
Active site of the replication protein of the rolling circle plasmid pC194.
MF Noirot‐Gros, V Bidnenko, SD Ehrlich - The EMBO Journal, 1994 - embopress.org
Mutation analysis of the rolling circle (RC) replication initiator protein RepA of plasmid
pC194 was targeted to tyrosine and acidic amino acids (glutamate and aspartate) which are …
pC194 was targeted to tyrosine and acidic amino acids (glutamate and aspartate) which are …
Phosphoryl transfer in Flp recombination: a template for strand transfer mechanisms
M Jayaram - Trends in biochemical sciences, 1994 - cell.com
The basic chemistry involved in DNA recombination, RNA splicing and DNA transposition is
a phosphoryl transfer reaction. This review is an attempt to provoke a unified thinking on the …
a phosphoryl transfer reaction. This review is an attempt to provoke a unified thinking on the …
Lambda integrase cleaves DNA in cis.
SE Nunes‐Düby, RS Tirumalai, L Dorgai, E Yagil… - The EMBO …, 1994 - embopress.org
In the Int family of site‐specific recombinases, DNA cleavage is accomplished by
nucleophilic attack on the activated scissile phosphodiester bond by a specific tyrosine …
nucleophilic attack on the activated scissile phosphodiester bond by a specific tyrosine …
Use of site-specific recombination systems in plants
JT Odell, SH Russell - Homologous recombination and gene silencing in …, 1994 - Springer
11. Use of Site-Specific Recombination Systems in Plants Page 1 11. Use of Site-Specific
Recombination Systems in Plants JOAN T. ODELL and SANDRA H. RUSSELL Contents …
Recombination Systems in Plants JOAN T. ODELL and SANDRA H. RUSSELL Contents …
Site‐Specific Recombination by the Flp Protein of Saccharomyces cerevisiae
This chapter reviews the biochemical, mechanistic, and topological aspects of the Flp
system with special emphasis on the contributions made by the Flp protein subunits and the …
system with special emphasis on the contributions made by the Flp protein subunits and the …
Analyses of the first chemical step in Flp site-specific recombination: synapsis may not be a pre-requisite for strand cleavage
The site-specific recombination reaction mediated by the Flp recombinase occurs within a
protein-DNA complex containing four monomers of Flp and two DNA substrates. The …
protein-DNA complex containing four monomers of Flp and two DNA substrates. The …
Reaction mechanism of T4 endonuclease V determined by analysis using modified oligonucleotide duplexes
S Iwai, M Maeda, M Shirai, Y Shimada, T Osafune… - Biochemistry, 1995 - ACS Publications
Revised Manuscript Received January 24, 7995® abstract: The reaction mechanism of
bacteriophage T4 endonuclease V was investigated using modified …
bacteriophage T4 endonuclease V was investigated using modified …
The yeast site-specific recombinase Flp mediates alcoholysis and hydrolysis of the strand cleavage product: mimicking the strand-joining reaction with non-DNA …
BR Knudsen, K Dahlstrøm, O Westergaard… - Journal of molecular …, 1997 - Elsevier
The yeast site-specific recombinase Flp is covalently linked to DNA via a 3′-phosphotyrosyl
bond during the strand-breakage step of recombination. We show that this phosphotyrosyl …
bond during the strand-breakage step of recombination. We show that this phosphotyrosyl …
Electrostatic suppression allows tyrosine site-specific recombination in the absence of a conserved catalytic arginine
PA Rowley, AH Kachroo, CH Ma, AD Maciaszek… - Journal of Biological …, 2010 - ASBMB
The active site of the tyrosine family site-specific recombinase Flp contains a conserved
catalytic pentad that includes two arginine residues, Arg-191 and Arg-308. Both arginines …
catalytic pentad that includes two arginine residues, Arg-191 and Arg-308. Both arginines …