Superresolution structured illumination microscopy reconstruction algorithms: a review

X Chen, S Zhong, Y Hou, R Cao, W Wang… - Light: Science & …, 2023 - nature.com
Structured illumination microscopy (SIM) has become the standard for next-generation wide-
field microscopy, offering ultrahigh imaging speed, superresolution, a large field-of-view …

Navigating challenges in the application of superresolution microscopy

TJ Lambert, JC Waters - Journal of Cell Biology, 2017 - rupress.org
In 2014, the Nobel Prize in Chemistry was awarded to three scientists who have made
groundbreaking contributions to the field of superresolution (SR) microscopy (SRM). The first …

Dendritic spine geometry and spine apparatus organization govern the spatiotemporal dynamics of calcium

M Bell, T Bartol, T Sejnowski… - Journal of General …, 2019 - rupress.org
Dendritic spines are small subcompartments that protrude from the dendrites of neurons and
are important for signaling activity and synaptic communication. These subcompartments …

SigmaR1 shapes rough endoplasmic reticulum membrane sheets

EM Sawyer, LE Jensen, JB Meehl, KP Larsen… - Developmental Cell, 2024 - cell.com
Rough endoplasmic reticulum (ER) sheets are a fundamental domain of the ER and the
gateway into the secretory pathway. Although reticulon proteins stabilize high-curvature ER …

Intrinsically disordered region-mediated condensation of IFN-inducible SCOTIN/SHISA-5 inhibits ER-to-Golgi vesicle transport

N Kim, TH Kim, C Kim, JE Lee, MG Kang, S Shin… - Developmental Cell, 2023 - cell.com
Newly synthesized proteins in the endoplasmic reticulum (ER) are sorted by coat protein
complex II (COPII) at the ER exit site en route to the Golgi. Under cellular stresses, COPII …

The REEP5/TRAM1 complex binds SARS-CoV-2 NSP3 and promotes virus replication

J Li, Q Gui, FX Liang, J Sall, Q Zhang, Y Duan… - Journal of …, 2023 - Am Soc Microbiol
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), like other coronaviruses,
replicates their genome in virus-induced cytosolic membrane-bound replication organelles …

The flexibility and dynamics of the tubules in the endoplasmic reticulum

P Georgiades, VJ Allan, GD Wright, PG Woodman… - Scientific reports, 2017 - nature.com
The endoplasmic reticulum (ER) is a single organelle in eukaryotic cells that extends
throughout the cell and is involved in a large number of cellular functions. Using a …

Unravelling the mystery inside cells by using single-molecule fluorescence imaging

J Zalejski, J Sun, A Sharma - Journal of Imaging, 2023 - mdpi.com
Live-cell imaging is a powerful technique to study the dynamics and mechanics of various
biological molecules like proteins, organelles, DNA, and RNA. With the rapid evolution of …

The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situ

LA Fuentes, Z Marin, J Tyson, D Baddeley… - Journal of Cell …, 2023 - rupress.org
The endoplasmic reticulum's (ER's) structure is directly linked to the many functions of the
ER, but its formation is not fully understood. We investigate how the ER–membrane curving …

Endoplasmic reticulum morphology regulation by RTN4 modulates neuronal regeneration by curbing luminal transport

T Konno, P Parutto, CC Crapart, V Davì, DMD Bailey… - Cell Reports, 2024 - cell.com
Cell functions rely on intracellular transport systems distributing bioactive molecules with
high spatiotemporal accuracy. The endoplasmic reticulum (ER) tubular network constitutes a …