FRET as a biomolecular research tool—understanding its potential while avoiding pitfalls

WR Algar, N Hildebrandt, SS Vogel, IL Medintz - Nature methods, 2019 - nature.com
The applications of Förster resonance energy transfer (FRET) grow with each year.
However, different FRET techniques are not applied consistently, nor are results uniformly …

A guide to fluorescent protein FRET pairs

BT Bajar, ES Wang, S Zhang, MZ Lin, J Chu - Sensors, 2016 - mdpi.com
Förster or fluorescence resonance energy transfer (FRET) technology and genetically
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …

EGFR targeting for cancer therapy: Pharmacology and immunoconjugates with drugs and nanoparticles

E da Silva Santos, KAB Nogueira… - International journal of …, 2021 - Elsevier
The epidermal growth factor receptor (EGFR) belongs to the tyrosine kinase receptors family
and is present in the epithelial cell membrane. Its endogenous activation occurs through the …

[HTML][HTML] Advanced fluorescence microscopy techniques—Frap, Flip, Flap, Fret and flim

HC Ishikawa-Ankerhold, R Ankerhold, GPC Drummen - Molecules, 2012 - mdpi.com
Fluorescence microscopy provides an efficient and unique approach to study fixed and
living cells because of its versatility, specificity, and high sensitivity. Fluorescence …

[图书][B] FRET-Förster resonance energy transfer: from theory to applications

IL Medintz, N Hildebrandt - 2013 - books.google.com
FRET–Förster Resonance Energy Transfer Meeting the need for an up-to-date and detailed
primer on all aspects of the topic, this ready reference reflects the incredible expansion in …

Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments

K Suhling, LM Hirvonen, JA Levitt, PH Chung… - Medical photonics, 2015 - Elsevier
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Activation of the EGF receptor by ligand binding and oncogenic mutations: the “rotation model”

ER Purba, E Saita, IN Maruyama - Cells, 2017 - mdpi.com
The epidermal growth factor receptor (EGFR) plays vital roles in cellular processes including
cell proliferation, survival, motility, and differentiation. The dysregulated activation of the …

Mechanisms of activation of receptor tyrosine kinases: monomers or dimers

IN Maruyama - Cells, 2014 - mdpi.com
Receptor tyrosine kinases (RTKs) play essential roles in cellular processes, including
metabolism, cell-cycle control, survival, proliferation, motility and differentiation. RTKs are all …

Fluorescence lifetime imaging microscopy in life sciences

JW Borst, AJWG Visser - Measurement Science and Technology, 2010 - iopscience.iop.org
Fluorescence lifetime imaging microscopy (FLIM) and fluorescence anisotropy imaging
microscopy (FAIM) are versatile tools for the investigation of the molecular environment of …

New insights for biosensing: Lessons from microbial defense systems

Y Wan, C Zong, X Li, A Wang, Y Li, T Yang… - Chemical …, 2022 - ACS Publications
Microorganisms have gained defense systems during the lengthy process of evolution over
millions of years. Such defense systems can protect them from being attacked by invading …