This Review addresses methods for obtaining sequence information directly from
unamplified genomic length DNA. Our generic starting point is a large piece of DNA that …

Abstract Theory and numerical simulations play a major role in the development of improved
and novel separation methods. In some cases, computer simulations predict counterintuitive …

Dielectrophoretic trapping of molecules is typically carried out using metal electrodes to
provide high field gradients. In this paper we demonstrate dielectrophoretic trapping using …

The free‐draining properties of DNA normally make it impossible to separate nucleic acids
by free‐flow electrophoresis. However, little is known, either theoretically or experimentally …

The electrophoretic mobilities and diffusion coefficients of single-and double-stranded DNA
molecules up to 50 000 bases or base pairs in size have been analyzed, using mobilities …

DNA is a free‐draining polymer. This subtle but “unfortunate” property of highly charged
polyelectrolytes makes it impossible to separate nucleic acids by free‐flow electrophoresis …

Transcription activator-like effector (TALE) proteins are a class of programmable DNA-
binding proteins used extensively for gene editing. Despite recent progress, however, little is …

Partitioning of protein–DNA complexes from protein-unbound DNA is a key step in selection
of DNA aptamers. Conceptually, the partitioning step is characterized by two parameters …

The development of nanostructure devices has opened the door to new DNA separation
techniques and fundamental investigations. With advanced nanotechnologies, artificial gels …

Over the last two decades, the introduction of new methods such as pulsed‐field gel
electrophoresis and capillary array electrophoresis has made it possible to map and …