Antimicrobial resistance threatens a resurgence of life-threatening bacterial infections and
the potential demise of many aspects of modern medicine. Despite intensive drug discovery …

Antimicrobial resistance has been a global health challenge that threatens our ability to
control and treat life-threatening bacterial infections. Despite ongoing efforts to identify new …

Highlights•Antibiotic lethality involves processes downstream of primary target
inhibition.•Respiration and altered metabolism actively participate in antibiotic lethality.• …

Synthetic lethality (SL) occurs between two genes when the inactivation of either gene alone
has no effect on cell survival but the inactivation of both genes results in cell death. SL …

Salmonella enterica remains an important food borne pathogen in all regions of the world
with S. Typhimurium as one of the most frequent serovars causing food borne disease …

Antibiotic resistance has been highlighted by international organizations, including World
Health Organization, World Bank and United Nations, as one of the most relevant global …

Personalized medicine and therapies represent the goal of modern medicine, as drug
discovery strives to move away from one-cure-for-all and makes use of the various targets …

Background Pseudomonas aeruginosa often causes multidrug-resistant infections in
immunocompromised patients, and polymyxins are often used as the last-line therapy …

Conventional efforts to describe essential genes in bacteria have typically emphasized
nutrient-rich growth conditions. Of note, however, are the set of genes that become essential …

Different conditions and genomic contexts are known to have an impact on gene essentiality
and interactions. Synthetic lethal interactions occur when a combination of perturbations …