Kinetochores are molecular machines that power chromosome segregation during the
mitotic and meiotic cell divisions of all eukaryotes. Aristotle explains how we think we have …

Highlights•Cross-linking-mass spectrometry constitutes a chemical method to produce
protein complex structural model constraints.•A wide range of sample types are applicable …

INTRODUCTION Protein-protein interactions play critical roles in biology, but the structures
of many eukaryotic protein complexes are unknown, and there are likely many interactions …

Accurate chromosome segregation relies on kinetochores carrying out multiple functions,
including establishing and maintaining microtubule attachments, forming precise bi-oriented …

Kinetochores couple chromosomes to the mitotic spindle to segregate the genome during
cell division. An error correction mechanism drives the turnover of kinetochore-microtubule …

The conserved Ndc80 kinetochore complex, Ndc80c, is the principal link between mitotic
spindle microtubules and centromere-associated proteins. We used AlphaFold 2 (AF2) to …

Summary The Mps1 and Aurora B kinases regulate and monitor kinetochore attachment to
spindle microtubules during cell division, ultimately ensuring accurate chromosome …

Accurate segregation of chromosomes relies on the force-bearing capabilities of the
kinetochore to robustly attach chromosomes to dynamic microtubule tips. The human Ska …

Kinetochores connect mitotic-spindle microtubules with chromosomes, allowing microtubule
depolymerization to pull chromosomes apart during anaphase while resisting detachment …

Forcing budding yeast to chromatinize their DNA with human histones manifests an abrupt
fitness cost. We previously proposed chromosomal aneuploidy and missense mutations as …