We present a protocol for the generation of a gene-deletion allelic-exchange plasmid and its
recovery in Escherichia coli for the purpose of constructing an in-frame gene deletion in …

Here, we describe a protocol for a scaled-down version of a genomic DNA (gDNA)-
fragmentation and tagmentation reaction using the Illumina Nextera XT DNA Library …

Here, we discuss methods for the selection of antibiotic-resistant bacteria and the use of
high-throughput whole-genome sequencing for the identification of the underlying …

Gene deletions can be generated in Staphylococcus aureus using recombineering in
combination with a CRISPR–Cas9 counterselection approach. The method involves first …

This protocol continues a series of methods for the construction of an in-frame gene deletion
in Staphylococcus aureus strain RN4220. To this end, we describe in this protocol an allelic …

Identifying the molecular mechanisms underlying antibiotic resistance is important, as it can
reveal key information on the mode of action of a drug and provide insights for the …

Methods for gene disruption are essential for functional genomics, and there are multiple
approaches for altering gene function in bacteria. One of these methods involves introducing …