Reading, writing and erasing mRNA methylation

S Zaccara, RJ Ries, SR Jaffrey - Nature reviews Molecular cell biology, 2019 - nature.com
RNA methylation to form N 6-methyladenosine (m6A) in mRNA accounts for the most
abundant mRNA internal modification and has emerged as a widespread regulatory …

Accurate detection of m6A RNA modifications in native RNA sequences

H Liu, O Begik, MC Lucas, JM Ramirez… - Nature …, 2019 - nature.com
The epitranscriptomics field has undergone an enormous expansion in the last few years;
however, a major limitation is the lack of generic methods to map RNA modifications …

Limits in the detection of m6A changes using MeRIP/m6A-seq

ABR McIntyre, NS Gokhale, L Cerchietti, SR Jaffrey… - Scientific reports, 2020 - nature.com
Many cellular mRNAs contain the modified base m6A, and recent studies have suggested
that various stimuli can lead to changes in m6A. The most common method to map m6A and …

Small changes, big implications: the impact of m6A RNA methylation on gene expression in pluripotency and development

AM Heck, CJ Wilusz - Biochimica et Biophysica Acta (BBA)-Gene …, 2019 - Elsevier
In order to maintain a state of self-renewal, yet retain the ability to rapidly differentiate in
response to external signals, pluripotent cells exert tight control over gene expression at …

Transcriptome-wide dynamics of extensive m6A mRNA methylation during Plasmodium falciparum blood-stage development

S Baumgarten, JM Bryant, A Sinha, T Reyser… - Nature …, 2019 - nature.com
Malaria pathogenesis results from the asexual replication of Plasmodium falciparum within
human red blood cells, which relies on a precisely timed cascade of gene expression over a …

In Vitro Selection with a Site-Specifically Modified RNA Library Reveals the Binding Preferences of N6-Methyladenosine Reader Proteins

AE Arguello, RW Leach, RE Kleiner - Biochemistry, 2019 - ACS Publications
Epitranscriptomic RNA modifications can serve as recognition elements for the recruitment
of effector proteins (ie,“readers”) to modified transcripts. While these interactions play an …