BACKGROUND Cellular life depends on the ability of organisms to accurately duplicate and
transmit genetic information between generations. In bacteria, archaea, and eukaryotes, this …

New antibiotics with novel modes of action are required to combat the growing threat posed
by multi-drug resistant bacteria. Over the last decade, genome sequencing and other high …

Ring-shaped hexameric helicases are essential motor proteins that separate duplex nucleic
acid strands for DNA replication, recombination, and transcriptional regulation. Two …

Within the last 15 years, members of the bacterial genus Acinetobacter have risen from
relative obscurity to be among the most important sources of hospital-acquired infections …

Genome duplication requires the coordinated activity of a multi-component machine, the
replisome. In contrast to the background of metabolic diversity across the bacterial domain …

The initiation of chromosomal DNA replication starts at a replication origin, which in bacteria
is a discrete locus that contains DNA sequence motifs recognized by an initiator protein …

Threading of DNA through the central channel of a replicative ring helicase is known as
helicase loading, and is a pivotal event during replication initiation at replication origins …

Replicative helicases are essential ATPases that unwind DNA to initiate chromosomal
replication. While bacterial replicative DnaB helicases are hexameric, Helicobacter pylori …

Hexameric DnaB helicases are often loaded at DNA replication forks by interacting with the
initiator protein DnaA and/or a helicase loader (DnaC in Escherichia coli). These loaders are …

Bacterial DNA replication requires DnaA, an AAA+ ATPase that initiates replication at a
specific chromosome region, oriC, and is regulated by species-specific regulators that …