Phase transitions of associative biomacromolecules
Multivalent proteins and nucleic acids, collectively referred to as multivalent associative
biomacromolecules, provide the driving forces for the formation and compositional …
biomacromolecules, provide the driving forces for the formation and compositional …
The molecular basis for cellular function of intrinsically disordered protein regions
AS Holehouse, BB Kragelund - Nature Reviews Molecular Cell Biology, 2024 - nature.com
Intrinsically disordered protein regions exist in a collection of dynamic interconverting
conformations that lack a stable 3D structure. These regions are structurally heterogeneous …
conformations that lack a stable 3D structure. These regions are structurally heterogeneous …
Expanding the molecular language of protein liquid–liquid phase separation
Understanding the relationship between a polypeptide sequence and its phase separation
has important implications for analysing cellular function, treating disease and designing …
has important implications for analysing cellular function, treating disease and designing …
Sequence-dependent material properties of biomolecular condensates and their relation to dilute phase conformations
D Sundaravadivelu Devarajan, J Wang… - Nature …, 2024 - nature.com
Material properties of phase-separated biomolecular condensates, enriched with disordered
proteins, dictate many cellular functions. Contrary to the progress made in understanding the …
proteins, dictate many cellular functions. Contrary to the progress made in understanding the …
Single-molecule FRET unmasks structural subpopulations and crucial molecular events during FUS low-complexity domain phase separation
Biomolecular condensates formed via phase separation of proteins and nucleic acids are
thought to be associated with a wide range of cellular functions and dysfunctions. We dissect …
thought to be associated with a wide range of cellular functions and dysfunctions. We dissect …
Thermodynamic forces from protein and water govern condensate formation of an intrinsically disordered protein domain
S Mukherjee, LV Schäfer - Nature Communications, 2023 - nature.com
Liquid-liquid phase separation (LLPS) can drive a multitude of cellular processes by
compartmentalizing biological cells via the formation of dense liquid biomolecular …
compartmentalizing biological cells via the formation of dense liquid biomolecular …
Adenosine triphosphate mediates phase separation of disordered basic proteins by bridging intermolecular interaction networks
Adenosine triphosphate (ATP) is an abundant molecule with crucial cellular roles as the
energy currency and a building block of nucleic acids and for protein phosphorylation. Here …
energy currency and a building block of nucleic acids and for protein phosphorylation. Here …
Sequence-specific interactions determine viscoelasticity and ageing dynamics of protein condensates
Biomolecular condensates are viscoelastic materials. Here we investigate the determinants
of the sequence-encoded and age-dependent viscoelasticity of condensates formed by the …
of the sequence-encoded and age-dependent viscoelasticity of condensates formed by the …
Driving forces of the complex formation between highly charged disordered proteins
Highly disordered complexes between oppositely charged intrinsically disordered proteins
present a new paradigm of biomolecular interactions. Here, we investigate the driving forces …
present a new paradigm of biomolecular interactions. Here, we investigate the driving forces …
Fluorescence resonance energy transfer at the single-molecule level
Fluorescence resonance energy transfer (FRET) is a powerful spectroscopic method for
measuring distances in the 2–8 nm range. Often, conformational changes and molecular …
measuring distances in the 2–8 nm range. Often, conformational changes and molecular …