Cloning and Direct Evolution of a Novel 7-O-Glycosyltransferase from Cucurbita moschata and Its Application in the Efficient Biocatalytic Synthesis of Luteolin-7-O …

C Lu, H Wang, D Zheng, S Jia, Q Xing… - Journal of Agricultural …, 2024 - ACS Publications
Luteolin-7-O-glucoside (L7G), a glycosylation product of luteolin, is present in a variety of
foods, vegetables, and medicinal herbs and is commonly used in dietary supplements due …

Catalytic efficiency and thermal stability promotion of the cassava linamarase with multiple mutations for better cyanogenic glycoside degradation

Y Zhong, Y Li, Q Chen, S Ji, M Xu, Y Liu, X Wu… - International Journal of …, 2023 - Elsevier
In our previous study, we found that cassava cyanogenic glycosides had an acute health
risk. Therefore, to solve this problem, the improvement of specific degradation of cyanogenic …

The main protease 3CLpro of the SARS-CoV-2 virus: how to turn an enemy into a helper

SV Belenkaya, IA Merkuleva, OI Yarovaya… - … in Bioengineering and …, 2023 - frontiersin.org
Despite the long history of use and the knowledge of the genetics and biochemistry of E.
coli, problems are still possible in obtaining a soluble form of recombinant proteins in this …

Engineering an optimized expression operating unit for improved recombinant protein production in Escherichia coli

SPO Santos, LFS Garcés, FSR Silva… - Protein Expression and …, 2022 - Elsevier
Common strategies to improve recombinant protein production in Escherichia coli often
involve the test and optimization of several different variables, when using traditional …

Soluble expression of hMYDGF was improved by strain engineering and optimizations of fermentation strategies in Escherichia coli

S Su, YS Zheng, H Mao, L Zhao, M Zhu, Y Yang… - Protein Expression and …, 2024 - Elsevier
Myeloid-derived growth factor (MYDGF) is a cytokine that exhibits a variety of biological
functions. This study focused on utilizing BL21 (DE3) strain engineering and fermentation …

[HTML][HTML] Production of mutant streptavidin protein and investigation of its effect on the performance of streptavidin

E Didevara, SS Abbasian, A Sadeghi… - Iranian Journal of Basic …, 2023 - ncbi.nlm.nih.gov
Objective (s): Streptavidin is a versatile protein in cell science. The tetramer structure of
streptavidin plays a key role in this binding, but this form interferes with some assays. If …