Reaction mechanisms of thiamin diphosphate enzymes: defining states of ionization and tautomerization of the cofactor at individual steps
We summarize the currently available information regarding the state of ionization and
tautomerization of the 4′‐aminopyrimidine ring of the thiamine diphosphate on enzymes …
tautomerization of the 4′‐aminopyrimidine ring of the thiamine diphosphate on enzymes …
Bacterial mandelic acid degradation pathway and its application in biotechnology
Q Wang, S Geng, L Wang, Z Wen… - Journal of Applied …, 2022 - academic.oup.com
Mandelic acid and its derivatives are an important class of chemical synthetic blocks, which
is widely used in drug synthesis and stereochemistry research. In nature, mandelic acid …
is widely used in drug synthesis and stereochemistry research. In nature, mandelic acid …
Catalysis in Enzymatic Decarboxylations: Comparison of Selected Cofactor-dependent and Cofactor-independent Examples.
F Jordan, H Patel - ACS catalysis, 2013 - ACS Publications
This review is focused on three types of enzymes decarboxylating very different
substrates:(1) thiamin diphosphate (ThDP)-dependent enzymes reacting with 2-oxo …
substrates:(1) thiamin diphosphate (ThDP)-dependent enzymes reacting with 2-oxo …
Substrate specificity in thiamin diphosphate-dependent decarboxylases
FH Andrews, MJ McLeish - Bioorganic chemistry, 2012 - Elsevier
Thiamin diphosphate (ThDP) is the biologically active form of vitamin B1, and ThDP-
dependent enzymes are found in all forms of life. The catalytic mechanism of this family …
dependent enzymes are found in all forms of life. The catalytic mechanism of this family …
Revealing donor substrate-dependent mechanistic control on DXPS, an enzyme in bacterial central metabolism
ML Johnston, CL Freel Meyers - Biochemistry, 2021 - ACS Publications
The thiamin diphosphate-dependent enzyme 1-deoxy-d-xylulose 5-phosphate synthase
(DXPS) catalyzes the formation of DXP from pyruvate (donor) and d-glyceraldehyde 3 …
(DXPS) catalyzes the formation of DXP from pyruvate (donor) and d-glyceraldehyde 3 …
The Catalytic Mechanism of Human Transketolase
We have computationally determined the catalytic mechanism of human transketolase (hTK)
using a cluster model approach and density functional theory calculations. We were able to …
using a cluster model approach and density functional theory calculations. We were able to …
Experimental observation of thiamin diphosphate-bound intermediates on enzymes and mechanistic information derived from these observations
F Jordan, NS Nemeria - Bioorganic Chemistry, 2005 - Elsevier
Thiamin diphosphate (ThDP), the vitamin B1 coenzyme, is an excellent representative of
coenzymes, which carry out electrophilic catalysis by forming a covalent complex with their …
coenzymes, which carry out electrophilic catalysis by forming a covalent complex with their …
Progress in the experimental observation of thiamin diphosphate-bound intermediates on enzymes and mechanistic information derived from these observations
F Jordan, NS Nemeria - Bioorganic chemistry, 2014 - Elsevier
Thiamin diphosphate (ThDP), the vitamin B1 coenzyme is an excellent representative of
coenzymes, which carry out electrophilic catalysis by forming a covalent complex with their …
coenzymes, which carry out electrophilic catalysis by forming a covalent complex with their …
QM/MM study of human transketolase: thiamine diphosphate activation mechanism and complete catalytic cycle
L Nauton, L Hecquet, V Théry - Journal of Chemical Information …, 2021 - ACS Publications
A computational model for human transketolase was proposed, showing that thiamine
diphosphate activation was based on His110 in place of His481 reported in yeast …
diphosphate activation was based on His110 in place of His481 reported in yeast …
A dual conformation of the post-decarboxylation intermediate is associated with distinct enzyme states in mycobacterial KGD (α-ketoglutarate decarboxylase)
T Wagner, N Barilone, PM Alzari… - Biochemical …, 2014 - portlandpress.com
α-Ketoacid dehydrogenases are large multi-enzyme machineries that orchestrate the
oxidative decarboxylation of α-ketoacids with the concomitant production of acyl-CoA and …
oxidative decarboxylation of α-ketoacids with the concomitant production of acyl-CoA and …