Dynamic nuclear polarization for sensitivity enhancement in biomolecular solid-state NMR

T Biedenbänder, V Aladin, S Saeidpour… - Chemical …, 2022 - ACS Publications
Solid-state NMR with magic-angle spinning (MAS) is an important method in structural
biology. While NMR can provide invaluable information about local geometry on an atomic …

[HTML][HTML] Dynamic nuclear polarization for sensitivity enhancement in modern solid-state NMR

ASL Thankamony, JJ Wittmann, M Kaushik… - Progress in nuclear …, 2017 - Elsevier
The field of dynamic nuclear polarization has undergone tremendous developments and
diversification since its inception more than 6 decades ago. In this review we provide an in …

Solid-state NMR spectroscopy

B Reif, SE Ashbrook, L Emsley, M Hong - Nature Reviews Methods …, 2021 - nature.com
Solid-state nuclear magnetic resonance (NMR) spectroscopy is an atomic-level method to
determine the chemical structure, 3D structure and dynamics of solids and semi-solids. This …

Biomolecular and biological applications of solid-state NMR with dynamic nuclear polarization enhancement

WY Chow, G De Paëpe, S Hediger - Chemical Reviews, 2022 - ACS Publications
Solid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables the
investigation of biological systems within their native context, such as lipid membranes, viral …

Structural insights into ion conduction by channelrhodopsin 2

O Volkov, K Kovalev, V Polovinkin, V Borshchevskiy… - Science, 2017 - science.org
INTRODUCTION Ion channels are integral membrane proteins that upon stimulation
modulate the flow of ions across the cell or organelle membrane. The resulting electrical …

BDPA-nitroxide biradicals tailored for efficient dynamic nuclear polarization enhanced solid-state NMR at magnetic fields up to 21.1 T

D Wisser, G Karthikeyan, A Lund… - Journal of the …, 2018 - ACS Publications
Dynamic nuclear polarization (DNP) solid-state nuclear magnetic resonance (NMR) has
developed into an invaluable tool for the investigation of a wide range of materials …

Role of computational methods in going beyond X-ray crystallography to explore protein structure and dynamics

A Srivastava, T Nagai, A Srivastava… - International journal of …, 2018 - mdpi.com
Protein structural biology came a long way since the determination of the first three-
dimensional structure of myoglobin about six decades ago. Across this period, X-ray …

Concepts and methods of solid-state NMR spectroscopy applied to biomembranes

TR Molugu, S Lee, MF Brown - Chemical reviews, 2017 - ACS Publications
Concepts of solid-state NMR spectroscopy and applications to fluid membranes are
reviewed in this paper. Membrane lipids with 2H-labeled acyl chains or polar head groups …

Unifying photocycle model for light adaptation and temporal evolution of cation conductance in channelrhodopsin-2

J Kuhne, J Vierock, SA Tennigkeit… - Proceedings of the …, 2019 - National Acad Sciences
Although channelrhodopsin (ChR) is a widely applied light-activated ion channel, important
properties such as light adaptation, photocurrent inactivation, and alteration of the ion …

Gd (III) and Mn (II) complexes for dynamic nuclear polarization: small molecular chelate polarizing agents and applications with site-directed spin labeling of proteins

M Kaushik, T Bahrenberg, TV Can… - Physical Chemistry …, 2016 - pubs.rsc.org
We investigate complexes of two paramagnetic metal ions Gd3+ and Mn2+ to serve as
polarizing agents for solid-state dynamic nuclear polarization (DNP) of 1H, 13C, and 15N at …