In this review we describe label-free optical spectroscopy techniques which are able to non-
invasively measure the (bio) chemistry in biological systems. Raman spectroscopy uses …

Biological tissues are not uniquely composed of cells. A substantial part of their volume is
extracellular space, which is primarily filled by an intricate network of macromolecules …

The preparation, staining, visualization and interpretation of histological images of tissue is
well accepted as the gold standard process for the diagnosis of disease. These methods …

The discovery of the Raman effect in 1928 not only aided fundamental understanding about
the quantum nature of light and matter but also opened up a completely novel area of optics …

Raman spectroscopy has been recognized to be a powerful tool to study cells and tissues
because the method provides molecular information without external markers such as stains …

Second‐harmonic generation (SHG) microscopy is a fantastic tool for imaging collagen and
probing its hierarchical organization from molecular scale up to tissue architectural level. In …

We reexamined the cellular drug delivery mechanism by poly (lactic-co-glycolic acid)
nanoparticles (PLGA NPs) to determine their utility and limitations as an intracellular drug …

The gold standard for clinical diagnostics of tissues is immunofluorescence staining. Toxicity
of many fluorescent dyes precludes their application in vivo. Raman spectroscopy, a …

Because each nonlinear optical (NLO) imaging modality is sensitive to specific molecules or
structures, multimodal NLO imaging capitalizes the potential of NLO microscopy for studies …

In this study we present an image analysis methodology capable of quantifying
morphological changes in tissue collagen fibril organization caused by pathological …