Vibrational spectroscopy provides a direct route to the physicochemical characterization of
molecules. While both IR and Raman spectroscopy have been used for decades to provide …

The cyclisation of polypeptides can play a crucial role in exerting biological functions,
maintaining stability under harsh conditions and conferring proteolytic resistance, as …

Asparaginyl endopeptidases (AEP) are cysteine proteases found in mammalian and plant
cells. Several AEP isoforms from plant species were found to exhibit transpeptidase activity …

Asparaginyl endopeptidases (AEPs) are ideal for peptide and protein labeling. However,
because of the reaction reversibility, a large excess of labels or backbone modified …

HIV-1 Protease (HIV-1 PR) is one of the three enzymes essential for the replication process
of HIV-1 virus, which explains why it has been the main target for design of drugs against …

While the function of enzymes has been well-known to researchers for decades, the driving
force behind it is still a hotly debated topic. Herein, we report significant evidence for …

Our understanding of how enzymes work is coloured by static structure depictions where the
enzyme scaffold is presented as either immobile, or in equilibrium between well‐defined …

Peptide backbone cyclization is commonly observed in nature and is increasingly applied to
proteins and peptides to improve thermal and chemical stability and resistance to proteolytic …

While being one of the most popular reaction rate theories, the applicability of transition state
theory to the study of enzymatic reactions has been often challenged. The complex dynamic …

Isotopically labeled enzymes (denoted as “heavy” or “Born–Oppenheimer” enzymes) have
been used to test the role of protein dynamics in catalysis. The original idea was that the …