Affinity and Pseudo-Affinity Membrane Chromatography for Viral Vector and Vaccine Purifications: A Review
Several chromatographic approaches have been established over the last decades for the
production of pharmaceutically relevant viruses. Due to the large size of these products …
production of pharmaceutically relevant viruses. Due to the large size of these products …
Discovery of therapeutic antibodies targeting complex multi-spanning membrane proteins
AD Stephens, T Wilkinson - BioDrugs, 2024 - Springer
Complex integral membrane proteins, which are embedded in the cell surface lipid bilayer
by multiple transmembrane spanning polypeptides, encompass families of proteins that are …
by multiple transmembrane spanning polypeptides, encompass families of proteins that are …
[HTML][HTML] Production, purification and immunogenicity of Gag virus-like particles carrying SARS-CoV-2 components
AB Gashti, G Agbayani, S Hrapovic, N Nassoury… - Vaccine, 2024 - Elsevier
The virus-like particle (VLP) platform is a robust inducer of humoral and cellular immune
responses; hence, it has been used in vaccine development for several infectious diseases …
responses; hence, it has been used in vaccine development for several infectious diseases …
Digital Twin for Continuous Production of Virus-like Particles toward Autonomous Operation
A Hengelbrock, F Probst, S Baukmann, A Uhl… - ACS …, 2024 - ACS Publications
Lentiviral vector and virus-like particle (VLP) manufacturing have been published in fed-
batch upstream and batch downstream modes before. Batch downstream and continuous …
batch upstream and batch downstream modes before. Batch downstream and continuous …
[HTML][HTML] Heparin-affinity chromatography is a generic purification platform for chimeric gag VLPs displaying different viral surface antigens
AM Zollner, LG Ruiz, V Mayer, S Stohl, LA Jakob… - Separation and …, 2024 - Elsevier
Chimeric virus-like particles formed by the co-expression of HIV-1 gag capsid protein and
surface proteins from other viruses can be used as a platform for production of a wide range …
surface proteins from other viruses can be used as a platform for production of a wide range …
[HTML][HTML] Highly pure measles virus generated by combination of salt-active nuclease treatment and heparin affinity chromatography
V Mayer, F Steiner, A Jungbauer, PP Aguilar - Journal of Chromatography A, 2024 - Elsevier
Highly purified virus preparations are essential for accurate activity and potency
determination. This requires simple and efficient purification methods, especially in the early …
determination. This requires simple and efficient purification methods, especially in the early …
The effects of high shear rates on the average hydrodynamic diameter measured in biomimetic HIV Gag virus-like particle dispersions
HIV Gag virus-like particles (HIV Gag VLPs) are promising HIV vaccine candidates. In the
literature, they are often described as shear-sensitive particles, and authors usually …
literature, they are often described as shear-sensitive particles, and authors usually …
[引用][C] Advanced cell technologies: Making protein, cell, and gene therapies a reality
I Geada, A Roldão, MJ Betenbaugh… - Biotechnology and …, 2023 - Wiley Online Library
Researchers and engineers came together in Lisbon at the 27th Meeting of the European
Society for Animal Cell Technology (ESACT 2022), to discuss the latest advances in …
Society for Animal Cell Technology (ESACT 2022), to discuss the latest advances in …