Although genotyping errors affect most data and can markedly influence the biological conclusions of a study, they are too often neglected. Errors have various causes, but their …
MA Burns, BN Johnson, SN Brahmasandra… - Science, 1998 - science.org
A device was developed that uses microfabricated fluidic channels, heaters, temperature sensors, and fluorescence detectors to analyze nanoliter-size DNA samples. The device is …
P Van Eerdewegh, RD Little, J Dupuis, RG Del Mastro… - Nature, 2002 - nature.com
Asthma is a common respiratory disorder characterized by recurrent episodes of coughing, wheezing and breathlessness. Although environmental factors such as allergen exposure …
P This, A Jung, P Boccacci, J Borrego, R Botta… - Theoretical and Applied …, 2004 - Springer
In order to investigate the comparability of microsatellite profiles obtained in different laboratories, ten partners in seven countries analyzed 46 grape cultivars at six loci (VVMD5 …
JI Hoffman, W Amos - Molecular ecology, 2005 - Wiley Online Library
Microsatellite genotyping errors will be present in all but the smallest data sets and have the potential to undermine the conclusions of most downstream analyses. Despite this, little …
L Gitlin, S Karelsky, R Andino - Nature, 2002 - nature.com
Gene silencing mediated by double-stranded RNA (dsRNA) is a sequence-specific, highly conserved mechanism in eukaryotes. In plants, it serves as an antiviral defence …
As genotyping methods move ever closer to full automation, care must be taken to ensure that there is no equivalent rise in allele‐calling error rates. One clear source of error lies with …
S Jain, RK Jain, SR McCouch - Theoretical and Applied Genetics, 2004 - Springer
Genetic relationships among Indian aromatic and quality rice (Oryza sativa) germplasm were assessed using 30 fluorescently labeled rice microsatellite markers. The 69 rice …
Microsatellite data are widely used to test ecological and evolutionary hypotheses in wild populations. In this paper, we consider three typical sources of scoring errors capable of …