Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals
Cellular function is controlled through intricate networks of signals, which lead to the myriad
pathways governing cell fate. Fluorescent biosensors have enabled the study of these …
pathways governing cell fate. Fluorescent biosensors have enabled the study of these …
Studying sars-cov-2 with fluorescence microscopy
LV Putlyaeva, KA Lukyanov - International Journal of Molecular Sciences, 2021 - mdpi.com
The COVID-19 pandemic caused by SARS-CoV-2 coronavirus deeply affected the world
community. It gave a strong impetus to the development of not only approaches to …
community. It gave a strong impetus to the development of not only approaches to …
LIVE-PAINT allows super-resolution microscopy inside living cells using reversible peptide-protein interactions
We present LIVE-PAINT, a new approach to super-resolution fluorescent imaging inside live
cells. In LIVE-PAINT only a short peptide sequence is fused to the protein being studied …
cells. In LIVE-PAINT only a short peptide sequence is fused to the protein being studied …
The tectonic complex regulates membrane protein composition in the photoreceptor cilium
HM Truong, KO Cruz-Colón… - Nature …, 2023 - nature.com
The primary cilium is a signaling organelle with a unique membrane composition
maintained by a diffusional barrier residing at the transition zone. Many transition zone …
maintained by a diffusional barrier residing at the transition zone. Many transition zone …
Small peptide–protein interaction pair for genetically encoded, fixation compatible peptide-PAINT
Super-resolution microscopy via PAINT has been widely adopted in life sciences to
interrogate the nanoscale architecture of many cellular structures. However, obtaining …
interrogate the nanoscale architecture of many cellular structures. However, obtaining …
Optimized Coiled‐Coil Interactions for Multiplexed Peptide‐PAINT
AS Eklund, R Jungmann - Small, 2023 - Wiley Online Library
Super‐resolution microscopy has revolutionized how researchers characterize samples in
the life sciences in the last decades. Amongst methods employing single‐molecule …
the life sciences in the last decades. Amongst methods employing single‐molecule …
PAINT using proteins: A new brush for super‐resolution artists
PAINT (points accumulation for imaging in nanoscale topography) refers to methods that
achieve the sparse temporal labeling required for super‐resolution imaging by using …
achieve the sparse temporal labeling required for super‐resolution imaging by using …
[HTML][HTML] Site-specific protein labeling strategies for super-resolution microscopy
Super-resolution microscopy (SRM) has transformed our understanding of proteins'
subcellular organization and revealed cellular details down to nanometers, far beyond …
subcellular organization and revealed cellular details down to nanometers, far beyond …
Transient fluorescence labeling: Low affinity—high benefits
MM Perfilov, AS Gavrikov, KA Lukyanov… - International Journal of …, 2021 - mdpi.com
Fluorescent labeling is an established method for visualizing cellular structures and
dynamics. The fundamental diffraction limit in image resolution was recently bypassed with …
dynamics. The fundamental diffraction limit in image resolution was recently bypassed with …
Tectonic Complex Impedes Diffusion through the Ciliary Transition Zone to Ensure Proper Sorting of Membrane Proteins
HM Truong, KO Cruz-Colón, JY Martínez-Márquez… - bioRxiv, 2023 - biorxiv.org
The primary cilium is a signaling organelle with a unique membrane composition
maintained by a diffusional barrier residing at the transition zone. Many transition zone …
maintained by a diffusional barrier residing at the transition zone. Many transition zone …