Over 500 natural and synthetic amino acids have been genetically encoded in the last two
decades. Incorporating these noncanonical amino acids into proteins enables many …

Over the past two decades, genetic code expansion (GCE)-enabled methods for
incorporating noncanonical amino acids (ncAAs) into proteins have significantly advanced …

Despite the great promise of genetic code expansion technology to modulate structures and
functions of proteins, external addition of ncAAs is required in most cases and it often limits …

Antibodies, particularly of the immunoglobulin G (IgG) isotype, are a group of biomolecules
that are extensively used as affinity reagents for many applications in research, disease …

Aromatic compounds have broad applications and have been the target of biosynthetic
processes for several decades. New biomolecular engineering strategies have been …

Unnatural amino acids (UAAs) have gained significant attention in protein engineering and
drug development owing to their ability to introduce new chemical functionalities to proteins …

Rationale: Therapeutic antibody conjugates allow for the specific delivery of cytotoxic agents
or immune cells to tumors, thus enhancing the antitumor activity of these agents and …

The concept of genetic code expansion (GCE) has revolutionized the field of chemical and
synthetic biology, enabling the site-specific incorporation of noncanonical amino acids …

Abstract Genetic Code Expansion technology offers significant potential in incorporating
noncanonical amino acids into proteins at precise locations, allowing for the modulation of …

While most organisms utilize 20 canonical amino acid building blocks for protein synthesis,
adding additional candidates to the amino acid repertoire can greatly facilitate the …