It is not a coincidence that both chirality and noncovalent interactions are ubiquitous in
nature and synthetic molecular systems. Noncovalent interactivity between chiral molecules …

Allosteric communication between distant sites in proteins is central to biological regulation
but still poorly characterized, limiting understanding, engineering and drug development …

This review analyzes a development in biochemistry, enzymology and biotechnology that
originally came as a surprise. Following the establishment of directed evolution of …

Engineering proteins and enzymes with the desired functionality has broad applications in
molecular biology, biotechnology, biomedical sciences, health, and medicine. The vastness …

Lipids are critical modulators of membrane protein structure and function. However, it is
challenging to investigate the thermodynamics of protein–lipid interactions because lipids …

Structural and functional studies of the ATP-binding cassette transporter MsbA have
revealed two distinct lipopolysaccharide (LPS) binding sites: one located in the central cavity …

In an effort to investigate the molecular determinants of ligand recognition of the C‐terminal
SH2 domain of the SHP2 protein, we conducted extensive site‐directed mutagenesis and …

Transcription factors (TFs) bind regulatory DNA to control gene expression, and mutations to
either TFs or DNA can alter binding affinities to rewire regulatory networks and drive …

The notion that protein function is allosterically regulated by structural or dynamic changes
in proteins has been extensively investigated in several protein domains in isolation. In …

Proteins exist as dynamic conformational ensembles. Here we suggest that the propensities
of the conformations can be predictors of cell function. The conformational states that the …