Engineering robust production microbes for large-scale cultivation

M Wehrs, D Tanjore, T Eng, J Lievense, TR Pray… - Trends in …, 2019 - cell.com
Systems biology and synthetic biology are increasingly used to examine and modulate
complex biological systems. As such, many issues arising during scaling-up microbial …

Recovery of polyhydroxyalkanoates from single and mixed microbial cultures: A review

G Pagliano, P Galletti, C Samorì, A Zaghini… - … in bioengineering and …, 2021 - frontiersin.org
An overview of the main polyhydroxyalkanoates (PHA) recovery methods is here reported,
by considering the kind of PHA-producing bacteria (single bacterial strains or mixed …

Poly (hydroxyalkanoates): Production, applications and end-of-life strategies–life cycle assessment nexus

JK Muiruri, JCC Yeo, Q Zhu, E Ye… - ACS Sustainable …, 2022 - ACS Publications
The runaway production and consumption of oil-based plastics are key drivers of global
warming and the increased carbon footprint. Besides, most of this plastic debris ends up in …

Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440

KE Nelson, C Weinel, IT Paulsen… - Environmental …, 2002 - Wiley Online Library
Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been
certified as a biosafety host for the cloning of foreign genes. The bacterium also has …

Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates

MP Mezzina, MT Manoli, MA Prieto… - Biotechnology …, 2021 - Wiley Online Library
Growing environmental concern sparks renewed interest in the sustainable production of
(bio) materials that can replace oil‐derived goods. Polyhydroxyalkanoates (PHAs) are …

Isolation and purification of bacterial poly (3-hydroxyalkanoates)

N Jacquel, CW Lo, YH Wei, HS Wu, SS Wang - Biochemical engineering …, 2008 - Elsevier
The isolation and the purification of bacterial polyhydroxyalkanoates are the key step of the
process profitability in the fermentation system. That is why many scientists have studied this …

Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms

A Steinbüchel, T Lütke-Eversloh - Biochemical engineering journal, 2003 - Elsevier
Prokaryotes synthesize a wide range of different polyhydroxyalkanoic acids (PHA) and
accumulate these polyesters as insoluble inclusions in the cytoplasm for storage of carbon …

Strategies for recovery and purification of poly[(R)‐3‐hydroxyalkanoates] (PHA) biopolyesters from surrounding biomass

M Koller, H Niebelschütz… - Engineering in Life …, 2013 - Wiley Online Library
To provide competitive alternatives to classical plastics, production of poly [(R)‐3‐
hydroxyalkanoate](PHA) biopolyesters has to become more economical. Downstream …

Polyhydroxyalkanoate synthase (PhaC): The key enzyme for biopolyester synthesis

SZ Neoh, MF Chek, HT Tan, JA Linares-Pastén… - Current Research in …, 2022 - Elsevier
Polyhydroxyalkanoates (PHAs) are considered good candidates in replacing commercial
petrochemical plastics in certain applications like single-use packaging since they are …

Production of polyhydroxyalkanoates containing monomers conferring amorphous and elastomeric properties from renewable resources: Current status and future …

HM Song, JC Joo, SH Lim, HJ Lim, S Lee… - Bioresource Technology, 2022 - Elsevier
Petrochemical-based plastics cause environmental pollution and threaten humans and
ecosystems. Polyhydroxyalkanoate (PHA) is considered a promising alternative to …