Diverse biochemical, structural, and in vivo data support models for the regulation of
polycomb repressive complex 2 (PRC2) activity by RNAs, which may contribute to the …

Here, we expound on the view that Xist RNA directly controls Polycomb repressive complex
2 (PRC2) recruitment, off-loading to chromatin, catalytic activity, and eviction from chromatin …

Abstract G-quadruplexes (G4s) are non-canonical nucleic acid secondary structures formed
by guanine-rich DNA or RNA sequences. These structures play pivotal roles in cellular …

X-chromosome inactivation is triggered by the long non-coding RNA XIST, whose structure
is characterized by tandem repeats that modularly recruit different proteins and chromatin …

RNAs are increasingly recognized as promising therapeutic targets, susceptible to
modulation by strategies that include targeting with small molecules, antisense …

Methyltransferase PRC2 (Polycomb Repressive Complex 2) introduces histone H3K27
trimethylation, a repressive chromatin mark, to tune the differential expression of genes …

RNA plays important roles in the regulation of gene expression in response to
environmental stimuli. SVALKA, a long noncoding cis-natural antisense RNA, is a key …

Using halo-tagged PRC2 and “CLAP” methodology, Guo et al. recently came to the
conclusion that PRC2 is not an RNA binding protein (RBP). They suggested that previous …

Here, we present a protocol for using d-rG4-seq, a technique for mapping RNA G-
quadruplex (rG4) for chromatin-bound RNA. We describe steps for identifying in vivo rG4 …

X-chromosome inactivation (XCI) is an essential process in eutherian mammals to
compensate dosage between females and males. XCI is triggered by XIST, a long non …