Polyhydroxyalkanoate (PHA): Applications in drug delivery and tissue engineering

E Elmowafy, A Abdal-Hay, A Skouras… - Expert review of …, 2019 - Taylor & Francis
Introduction: The applications of naturally obtained polymers are tremendously increased
due to them being biocompatible, biodegradable, environmentally friendly and renewable in …

[HTML][HTML] Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects

H Raschmanová, A Weninger, A Glieder, K Kovar… - Biotechnology …, 2018 - Elsevier
Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome
engineering, while also changing the speed and efficiency of metabolic engineering in …

A PP1–PP2A phosphatase relay controls mitotic progression

A Grallert, E Boke, A Hagting, B Hodgson, Y Connolly… - Nature, 2015 - nature.com
The widespread reorganization of cellular architecture in mitosis is achieved through
extensive protein phosphorylation, driven by the coordinated activation of a mitotic kinase …

[HTML][HTML] Cellular factories for coenzyme Q10 production

SQE Lee, TS Tan, M Kawamukai, ES Chen - Microbial cell factories, 2017 - Springer
Abstract Coenzyme Q 10 (CoQ 10), a benzoquinone present in most organisms, plays an
important role in the electron-transport chain, and its deficiency is associated with various …

Strategies for enhancing the homology-directed repair efficiency of CRISPR-cas systems

W Sun, H Liu, W Yin, J Qiao, X Zhao, Y Liu - The CRISPR journal, 2022 - liebertpub.com
The CRISPR-Cas nuclease has emerged as a powerful genome-editing tool in recent years.
The CRISPR-Cas system induces double-strand breaks that can be repaired via the non …

A toolbox of stable integration vectors in the fission yeast Schizosaccharomyces pombe

A Vještica, M Marek, PJ Nkosi, L Merlini… - Journal of cell …, 2020 - journals.biologists.com
Schizosaccharomyces pombe is a widely used model organism to study many aspects of
eukaryotic cell physiology. Its popularity as an experimental system partially stems from the …

Near-infrared imaging in fission yeast using a genetically encoded phycocyanobilin biosynthesis system

K Sakai, Y Kondo, H Fujioka, M Kamiya… - Journal of Cell …, 2021 - journals.biologists.com
Near-infrared fluorescent protein (iRFP) is a bright and stable fluorescent protein with near-
infrared excitation and emission maxima. Unlike the other conventional fluorescent proteins …

[HTML][HTML] A molecular genetic toolbox for Yarrowia lipolytica

EL Bredeweg, KR Pomraning, Z Dai, J Nielsen… - Biotechnology for …, 2017 - Springer
Yarrowia lipolytica is an ascomycete yeast used in biotechnological research for its abilities
to secrete high concentrations of proteins and accumulate lipids. Genetic tools have been …

[HTML][HTML] Acute heat stress leads to reversible aggregation of nuclear proteins into nucleolar rings in fission yeast

P Gallardo, P Real-Calderón, I Flor-Parra, S Salas-Pino… - Cell reports, 2020 - cell.com
Upon acute heat stress (HS), overall mRNA transcription, processing, and export are
inhibited, leading to cell growth arrest. However, how cells turn off mRNA metabolism is not …

Established and upcoming yeast expression systems

B Gündüz Ergün, D Hüccetoğulları, S Öztürk… - … protein production in …, 2019 - Springer
Yeast was the first microorganism used by mankind for biotransformation of feedstock that
laid the foundations of industrial biotechnology. Long historical use, vast amount of data …