SUMMARY HIV-1 DNA exists in nonintegrated linear and circular episomal forms and as
integrated proviruses. In patients with plasma viremia, most peripheral blood mononuclear …

Population (Sanger) sequencing has been the standard method in basic and clinical DNA
sequencing for almost 40 years; however, next-generation (deep) sequencing …

Background Contemporary antiretroviral therapies (ART) and management strategies have
diminished both human immunodeficiency virus (HIV) treatment failure and the acquired …

Bictegravir (BIC) and cabotegravir (CAB) are the latest available HIV integrase inhibitors in
clinical trials. The combination of major integrase inhibitor substitutions G140S/Q148H has …

Antiretroviral therapy (ART) can sustain the suppression of plasma viremia to below
detection levels. Infected individuals undergoing a treatment interruption exhibit rapid viral …

Background In most patients, current antiretroviral therapy (ART) regimens can rapidly
reduce plasma viral load. However, even after years of effective treatment, a significant …

Objectives This study investigated strategies that may increase the yield of drug resistance
testing prior to starting antiretroviral therapy (ART), and whether transmitted and …

Background The current widely applied standard method to screen for HIV-1 genotypic
resistance is based on Sanger population sequencing (Sseq), which does not allow for the …

Background Using HIV proviral DNA as a template may be suitable for initial detection of
transmitted drug resistance mutations (TDRMs) as it is easy to handle and less expensive …

Abstract Background The Department of Health and Human Services HIV-1 Treatment
Guidelines recommend drug resistance testing in HIV-1 RNA to guide the selection of …