Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges

L Möckl, WE Moerner - Journal of the American Chemical …, 2020 - ACS Publications
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …

Cellular incorporation of unnatural amino acids and bioorthogonal labeling of proteins

K Lang, JW Chin - Chemical reviews, 2014 - ACS Publications
In the past 15 years, there have been rapid developments in the ability to cotranslationally
incorporate unnatural amino acids into proteins produced in cells, both at defined sites via …

General design strategy to precisely control the emission of fluorophores via a twisted intramolecular charge transfer (TICT) process

K Hanaoka, S Iwaki, K Yagi, T Myochin… - Journal of the …, 2022 - ACS Publications
Fluorogenic probes for bioimaging have become essential tools for life science and
medicine, and the key to their development is a precise understanding of the mechanisms …

A general method to optimize and functionalize red-shifted rhodamine dyes

JB Grimm, AN Tkachuk, L Xie, H Choi, B Mohar… - Nature …, 2020 - nature.com
Expanding the palette of fluorescent dyes is vital to push the frontier of biological imaging.
Although rhodamine dyes remain the premier type of small-molecule fluorophore owing to …

Single-molecule FRET imaging of GPCR dimers in living cells

WB Asher, P Geggier, MD Holsey, GT Gilmore… - Nature …, 2021 - nature.com
Class CG protein-coupled receptors (GPCRs) are known to form stable homodimers or
heterodimers critical for function, but the oligomeric status of class A and B receptors, which …

Nuclear pores as versatile reference standards for quantitative superresolution microscopy

JV Thevathasan, M Kahnwald, K Cieśliński, P Hoess… - Nature …, 2019 - nature.com
Quantitative fluorescence and superresolution microscopy are often limited by insufficient
data quality or artifacts. In this context, it is essential to have biologically relevant control …

A critical and comparative review of fluorescent tools for live-cell imaging

EA Specht, E Braselmann… - Annual review of …, 2017 - annualreviews.org
Fluorescent tools have revolutionized our ability to probe biological dynamics, particularly at
the cellular level. Fluorescent sensors have been developed on several platforms, utilizing …

Bright photoactivatable fluorophores for single-molecule imaging

JB Grimm, BP English, H Choi, AK Muthusamy… - Nature …, 2016 - nature.com
Small-molecule fluorophores are important tools for advanced imaging experiments. We
previously reported a general method to improve small, cell-permeable fluorophores which …

Single-virus tracking: from imaging methodologies to virological applications

SL Liu, ZG Wang, HY Xie, AA Liu, DC Lamb… - Chemical …, 2020 - ACS Publications
Uncovering the mechanisms of virus infection and assembly is crucial for preventing the
spread of viruses and treating viral disease. The technique of single-virus tracking (SVT) …

Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging

Q Zheng, AX Ayala, I Chung, AV Weigel… - ACS Central …, 2019 - ACS Publications
Rhodamine dyes exist in equilibrium between a fluorescent zwitterion and a nonfluorescent
lactone. Tuning this equilibrium toward the nonfluorescent lactone form can improve cell …