Local delivery of stabilized chondroitinase ABC degrades chondroitin sulfate proteoglycans in stroke-injured rat brains
Central nervous system (CNS) injuries, such as stroke and spinal cord injuries, result in the
formation of a proteoglycan-rich glial scar, which acts as a barrier to axonal regrowth and …
formation of a proteoglycan-rich glial scar, which acts as a barrier to axonal regrowth and …
Chondroitinase as a therapeutic enzyme: Prospects and challenges
The chondroitinases (Chase) are bacterial lyases that specifically digest chondroitin sulfate
and/or dermatan sulfate glycosaminoglycans via a β-elimination reaction and generate …
and/or dermatan sulfate glycosaminoglycans via a β-elimination reaction and generate …
Reengineering biocatalysts: Computational redesign of chondroitinase ABC improves efficacy and stability
Maintaining biocatalyst stability and activity is a critical challenge. Chondroitinase ABC
(ChABC) has shown promise in central nervous system (CNS) regeneration, yet its …
(ChABC) has shown promise in central nervous system (CNS) regeneration, yet its …
Enhancing myelin repair in experimental model of multiple sclerosis using immobilized chondroitinase ABC I on porous silicon nanoparticles
Removal of chondroitin sulfate proteoglycans (CSPGs) with chondroitinase ABC I (ChABC)
facilitates axonal plasticity, axonal regeneration and remyelination, following injury to the …
facilitates axonal plasticity, axonal regeneration and remyelination, following injury to the …
Rigidifying flexible sites: An approach to improve stability of chondroitinase ABC I
The stability of chondroitin ABC lyase I (cABC I) at physiological temperature is one of the
current obstacles to its clinical application. In this study, we used a protein engineering …
current obstacles to its clinical application. In this study, we used a protein engineering …
Engineering a thermostable chondroitinase for production of specifically distributed low‐molecular‐weight chondroitin sulfate
H Wang, L Zhang, Y Wang, J Li, G Du… - Biotechnology …, 2021 - Wiley Online Library
Abstract Chondroitinase ABC I (csABC I) has attracted intensive attention because of its
great potential in heparin refining and the enzymatic preparation of low‐molecular‐weight …
great potential in heparin refining and the enzymatic preparation of low‐molecular‐weight …
A comprehensive atlas of Aggrecan, Versican, Neurocan and Phosphacan expression across time in wildtype retina and in retinal degeneration
As photoreceptor cells die during retinal degeneration, the surrounding microenvironment
undergoes significant changes that are increasingly recognized to play a prominent role in …
undergoes significant changes that are increasingly recognized to play a prominent role in …
Improvement of activity and stability of Chondroitinase ABC I by introducing an aromatic cluster at the surface of protein
Abstract Chondroitinase ABC I (ChABC I) has been shown to depolymerize a variety of
glycosaminoglycan substrates and promote regeneration of damaged spinal cord. However …
glycosaminoglycan substrates and promote regeneration of damaged spinal cord. However …
Improving the stability of chondroitinase ABC I via interaction with gold nanorods
Abstract Chondroitinase ABC I (cABC I) cleaves glycosaminoglycan chains which are
responsible for most of the inhibition of axon regrowth in spinal cord injury. The application …
responsible for most of the inhibition of axon regrowth in spinal cord injury. The application …
Chondroitinase ABC/galectin-3 fusion proteins with hyaluronan-based hydrogels stabilize enzyme and provide targeted enzyme activity for neural applications
N Hlavac, DT Seroski, NK Agrawal… - Journal of Neural …, 2021 - iopscience.iop.org
Objective. Chondroitinase ABC (ChABC) has emerged as a promising therapeutic agent for
central nervous system regeneration. Despite multiple beneficial outcomes for regeneration …
central nervous system regeneration. Despite multiple beneficial outcomes for regeneration …