Digital PCR applications in the SARS-CoV-2/COVID-19 era: a roadmap for future outbreaks

R Nyaruaba, C Mwaliko, D Dobnik… - Clinical Microbiology …, 2022 - Am Soc Microbiol
The ongoing coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to a global public health …

Gene expression studies of reference genes for quantitative real-time PCR: an overview in insects

M Shakeel, A Rodriguez, UB Tahir, F Jin - Biotechnology letters, 2018 - Springer
Whenever gene expression is being examined, it is essential that a normalization process is
carried out to eliminate non-biological variations. The use of reference genes, such as …

YAP 5-methylcytosine modification increases its mRNA stability and promotes the transcription of exosome secretion-related genes in lung adenocarcinoma

W Yu, C Zhang, Y Wang, X Tian, Y Miao, F Meng… - Cancer Gene …, 2023 - nature.com
YAP is a transcriptional co-activator with critical roles in tumorigenesis. However, its
upstream regulatory mechanism, especially how its mRNA stability is regulated, remains to …

Quantification of environmental DNA (eDNA) shedding and decay rates for three commercially harvested fish species and comparison between eDNA detection and …

A Kirtane, D Wieczorek, T Noji, L Baskin… - Environmental …, 2021 - Wiley Online Library
Stock assessments are critical to inform decisions for sustainable fisheries management.
Environmental DNA (eDNA) analysis is a potential tool for assessing fish biomass and …

Development of a multiplex real-time PCR to differentiate the four major Listeria monocytogenes serotypes in isolates from meat processing plants

A Alía, MJ Andrade, JJ Córdoba, I Martín, A Rodríguez - Food Microbiology, 2020 - Elsevier
Listeria monocytogenes is an important foodborne pathogen, causative agent of listeriosis.
The epidemiology and persistence of this bacterium in meat processing plants may be …

Clade-Specific Biosurveillance of Pseudoperonospora cubensis Using Spore Traps for Precision Disease Management of Cucurbit Downy Mildew

A Rahman, JR Standish, KN D'arcangelo… - …, 2021 - Am Phytopath Society
Pseudoperonospora cubensis is an obligate oomycete and cause of cucurbit downy mildew
(CDM), the most destructive foliar disease affecting cucurbit hosts. Annual epidemics …

[HTML][HTML] Targeted non-invasive bioindicator species detection in eDNA water samples to assess and monitor the integrity of vulnerable alpine freshwater environments

L Blattner, JN Ebner, J Zopfi, S von Fumetti - Ecological indicators, 2021 - Elsevier
Due to a high number of specialized species and unique environmental conditions, alpine
spring ecosystems are particularly vulnerable to environmental change and human impact …

Suggested guidelines for validation of real-time PCR assays in veterinary diagnostic laboratories

K Toohey-Kurth, MM Reising… - Journal of …, 2020 - journals.sagepub.com
This consensus document presents the suggested guidelines developed by the Laboratory
Technology Committee (LTC) of the American Association of Veterinary Laboratory …

[HTML][HTML] A pipeline for the development and analysis of extracellular vesicle-based transcriptomic biomarkers in molecular diagnostics

C Grätz, M Schuster, F Brandes, AS Meidert… - Molecular Aspects of …, 2024 - Elsevier
Extracellular vesicles are shed by every cell type and can be found in any biofluid. They
contain different molecules that can be utilized as biomarkers, including several RNA …

[HTML][HTML] The impact of mosquito sampling strategies on molecular xenomonitoring prevalence for filariasis: a systematic review

LJ Reimer, JD Pryce - Bulletin of the World Health Organization, 2024 - ncbi.nlm.nih.gov
Objective To explore the impact of mosquito collection methods, sampling intensity and
target genus on molecular xenomonitoring detection of parasites causing lymphatic …