Fluorescent proteins and their applications in imaging living cells and tissues
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …
diverse marine animals are widely used as universal genetically encoded fluorescent labels …
Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells
TK Kerppola - Annu. Rev. Biophys., 2008 - annualreviews.org
Protein interactions are a fundamental mechanism for the generation of biological regulatory
specificity. The study of protein interactions in living cells is of particular significance …
specificity. The study of protein interactions in living cells is of particular significance …
Perception of UV-B by the Arabidopsis UVR8 Protein
To optimize their growth and survival, plants perceive and respond to ultraviolet-B (UV-B)
radiation. However, neither the molecular identity of the UV-B photoreceptor nor the …
radiation. However, neither the molecular identity of the UV-B photoreceptor nor the …
CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3
The prokaryotic CRISPR/Cas immune system is based on genomic loci that contain
incorporated sequence tags from viruses and plasmids. Using small guide RNA molecules …
incorporated sequence tags from viruses and plasmids. Using small guide RNA molecules …
Parkinson-causing α-synuclein missense mutations shift native tetramers to monomers as a mechanism for disease initiation
Abstract β-Sheet-rich α-synuclein (αS) aggregates characterize Parkinson's disease (PD).
αS was long believed to be a natively unfolded monomer, but recent work suggests it also …
αS was long believed to be a natively unfolded monomer, but recent work suggests it also …
Interaction of COP1 and UVR8 regulates UV‐B‐induced photomorphogenesis and stress acclimation in Arabidopsis
JJ Favory, A Stec, H Gruber, L Rizzini, A Oravecz… - The EMBO …, 2009 - embopress.org
The ultraviolet‐B (UV‐B) portion of the solar radiation functions as an environmental signal
for which plants have evolved specific and sensitive UV‐B perception systems. The UV‐B …
for which plants have evolved specific and sensitive UV‐B perception systems. The UV‐B …
Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta
R Waadt, LK Schmidt, M Lohse, K Hashimoto… - The Plant …, 2008 - Wiley Online Library
The specificity of intracellular signaling and developmental patterning in biological systems
relies on selective interactions between different proteins in specific cellular compartments …
relies on selective interactions between different proteins in specific cellular compartments …
GFP Reconstitution Across Synaptic Partners (GRASP) defines cell contacts and synapses in living nervous systems
EH Feinberg, MK VanHoven, A Bendesky, G Wang… - Neuron, 2008 - cell.com
The identification of synaptic partners is challenging in dense nerve bundles, where many
processes occupy regions beneath the resolution of conventional light microscopy. To …
processes occupy regions beneath the resolution of conventional light microscopy. To …
[HTML][HTML] Competing E3 ubiquitin ligases govern circadian periodicity by degradation of CRY in nucleus and cytoplasm
Period determination in the mammalian circadian clock involves the turnover rate of the
repressors CRY and PER. We show that CRY ubiquitination engages two competing E3 …
repressors CRY and PER. We show that CRY ubiquitination engages two competing E3 …
Crystal structure of the heterodimeric CLOCK: BMAL1 transcriptional activator complex
The circadian clock in mammals is driven by an autoregulatory transcriptional feedback
mechanism that takes approximately 24 hours to complete. A key component of this …
mechanism that takes approximately 24 hours to complete. A key component of this …