In animals, the sequences for controlling gene expression do not concentrate just at the
transcription start site of genes, but are frequently thousands to millions of base pairs distal …

Over a decade ago the advent of high-throughput chromosome conformation capture (Hi-C)
sparked a new era of 3D genomics. Since then the number of methods for mapping the 3D …

The outcome of CRISPR-Cas-mediated genome modifications is dependent on DNA double-
strand break (DSB) processing and repair pathway choice. Homology-directed repair (HDR) …

Nuclear speckles are nuclear membraneless organelles in higher eukaryotic cells playing a
vital role in gene expression. Using an in situ reverse transcription–based sequencing …

Fluorescence in situ hybridization (FISH) is the gold standard for visualizing genomic DNA in
fixed cells and tissues, but it is incompatible with live-cell imaging, and its combination with …

Owing to their unique abilities to manipulate, label, and image individual molecules in vitro
and in cellulo, single-molecule techniques provide previously unattainable access to …

The rapid and accurate identification of methicillin-resistant Staphylococcus aureus at an
early antibiotic therapy stage would be benefit to disease diagnosis and antibiotic selection …

DNA fluorescence in situ hybridization (FISH) has been a central technique in advancing our
understanding of how chromatin is organized within the nucleus. With the increasing …

Background Fluorescence in situ hybridization (FISH) is a technique that uses fluorescently
labeled DNA probes. Many studies have evaluated the ROC curve (sensitivity and …

Genomes are self-organized and self-maintained as long, complex macromolecules of
chromatin. The inherent heterogeneity, stochasticity, phase separation, and chromatin …