Recent advances in FRET for the study of protein interactions and dynamics

K Okamoto, Y Sako - Current opinion in structural biology, 2017 - Elsevier
Highlights•This review introduces three advanced FRET methodology in the
following.•Quantitative analysis of interaction parameters using phasor plot based on FLIM …

Supercontinuum radiation in fluorescence microscopy and biomedical imaging applications

C Poudel, CF Kaminski - JOSA B, 2019 - opg.optica.org
Compact, high brightness supercontinuum sources have already made a big impact in the
fields of fluorescence microscopy and biomedical imaging techniques, such as optical …

Machine learning for faster and smarter fluorescence lifetime imaging microscopy

V Mannam, Y Zhang, X Yuan, C Ravasio… - Journal of Physics …, 2020 - iopscience.iop.org
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique in biomedical
research that uses the fluorophore decay rate to provide additional contrast in fluorescence …

Translation elongation factor eEF1A2 is a novel anticancer target for the marine natural product plitidepsin

A Losada, MJ Muñoz-Alonso, C García… - Scientific reports, 2016 - nature.com
Abstract eEF1A2 is one of the isoforms of the alpha subunit of the eukaryotic Elongation
Factor 1. It is overexpressed in human tumors and is endowed with oncogenic properties …

Fluorescence self-quenching from reporter dyes informs on the structural properties of amyloid clusters formed in vitro and in cells

WY Chen, LJ Young, M Lu, A Zaccone, F Strohl… - Nano …, 2017 - ACS Publications
The characterization of the aggregation kinetics of protein amyloids and the structural
properties of the ensuing aggregates are vital in the study of the pathogenesis of many …

Advanced FRET normalization allows quantitative analysis of protein interactions including stoichiometries and relative affinities in living cells

B Hochreiter, M Kunze, B Moser, JA Schmid - Scientific reports, 2019 - nature.com
Abstract FRET (Fluorescence Resonance Energy Transfer) measurements are commonly
applied to proof protein-protein interactions. However, standard methods of live cell FRET …

Intramitochondrial proteostasis is directly coupled to α-synuclein and amyloid β1-42 pathologies

J Lautenschläger, S Wagner-Valladolid… - Journal of Biological …, 2020 - ASBMB
Mitochondrial dysfunction has long been implicated in the neurodegenerative disorder
Parkinson's disease (PD); however, it is unclear how mitochondrial impairment and α …

Determination of oligomerization state of Drp1 protein in living cells at nanomolar concentrations

K Kwapiszewska, T Kalwarczyk, B Michalska… - Scientific Reports, 2019 - nature.com
Biochemistry in living cells is an emerging field of science. Current quantitative bioassays
are performed ex vivo, thus equilibrium constants and reaction rates of reactions occurring in …

Fast and simple spectral FLIM for biochemical and medical imaging

M Popleteeva, KT Haas, D Stoppa, L Pancheri… - Optics express, 2015 - opg.optica.org
Spectrally resolved fluorescence lifetime imaging microscopy (λFLIM) has powerful potential
for biochemical and medical imaging applications. However, long acquisition times, low …

Phasor analysis of NADH FLIM identifies pharmacological disruptions to mitochondrial metabolic processes in the rodent cerebral cortex

CA Gómez, J Sutin, W Wu, B Fu, H Uhlirova, A Devor… - PLoS one, 2018 - journals.plos.org
Investigating cerebral metabolism in vivo at a microscopic level is essential for
understanding brain function and its pathological alterations. The intricate signaling and …