Recent advances in FRET for the study of protein interactions and dynamics
K Okamoto, Y Sako - Current opinion in structural biology, 2017 - Elsevier
Highlights•This review introduces three advanced FRET methodology in the
following.•Quantitative analysis of interaction parameters using phasor plot based on FLIM …
following.•Quantitative analysis of interaction parameters using phasor plot based on FLIM …
Supercontinuum radiation in fluorescence microscopy and biomedical imaging applications
C Poudel, CF Kaminski - JOSA B, 2019 - opg.optica.org
Compact, high brightness supercontinuum sources have already made a big impact in the
fields of fluorescence microscopy and biomedical imaging techniques, such as optical …
fields of fluorescence microscopy and biomedical imaging techniques, such as optical …
Machine learning for faster and smarter fluorescence lifetime imaging microscopy
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique in biomedical
research that uses the fluorophore decay rate to provide additional contrast in fluorescence …
research that uses the fluorophore decay rate to provide additional contrast in fluorescence …
Translation elongation factor eEF1A2 is a novel anticancer target for the marine natural product plitidepsin
A Losada, MJ Muñoz-Alonso, C García… - Scientific reports, 2016 - nature.com
Abstract eEF1A2 is one of the isoforms of the alpha subunit of the eukaryotic Elongation
Factor 1. It is overexpressed in human tumors and is endowed with oncogenic properties …
Factor 1. It is overexpressed in human tumors and is endowed with oncogenic properties …
Fluorescence self-quenching from reporter dyes informs on the structural properties of amyloid clusters formed in vitro and in cells
The characterization of the aggregation kinetics of protein amyloids and the structural
properties of the ensuing aggregates are vital in the study of the pathogenesis of many …
properties of the ensuing aggregates are vital in the study of the pathogenesis of many …
Advanced FRET normalization allows quantitative analysis of protein interactions including stoichiometries and relative affinities in living cells
Abstract FRET (Fluorescence Resonance Energy Transfer) measurements are commonly
applied to proof protein-protein interactions. However, standard methods of live cell FRET …
applied to proof protein-protein interactions. However, standard methods of live cell FRET …
Intramitochondrial proteostasis is directly coupled to α-synuclein and amyloid β1-42 pathologies
J Lautenschläger, S Wagner-Valladolid… - Journal of Biological …, 2020 - ASBMB
Mitochondrial dysfunction has long been implicated in the neurodegenerative disorder
Parkinson's disease (PD); however, it is unclear how mitochondrial impairment and α …
Parkinson's disease (PD); however, it is unclear how mitochondrial impairment and α …
Determination of oligomerization state of Drp1 protein in living cells at nanomolar concentrations
K Kwapiszewska, T Kalwarczyk, B Michalska… - Scientific Reports, 2019 - nature.com
Biochemistry in living cells is an emerging field of science. Current quantitative bioassays
are performed ex vivo, thus equilibrium constants and reaction rates of reactions occurring in …
are performed ex vivo, thus equilibrium constants and reaction rates of reactions occurring in …
Fast and simple spectral FLIM for biochemical and medical imaging
Spectrally resolved fluorescence lifetime imaging microscopy (λFLIM) has powerful potential
for biochemical and medical imaging applications. However, long acquisition times, low …
for biochemical and medical imaging applications. However, long acquisition times, low …
Phasor analysis of NADH FLIM identifies pharmacological disruptions to mitochondrial metabolic processes in the rodent cerebral cortex
Investigating cerebral metabolism in vivo at a microscopic level is essential for
understanding brain function and its pathological alterations. The intricate signaling and …
understanding brain function and its pathological alterations. The intricate signaling and …