Nonlinear magic: multiphoton microscopy in the biosciences

WR Zipfel, RM Williams, WW Webb - Nature biotechnology, 2003 - nature.com
Multiphoton microscopy (MPM) has found a niche in the world of biological imaging as the
best noninvasive means of fluorescence microscopy in tissue explants and living animals …

Multiphoton microscopy in biological research

RM Williams, WR Zipfel, WW Webb - Current opinion in chemical biology, 2001 - Elsevier
From its conception a decade ago, multiphoton microscopy has evolved from a photonic
novelty to an indispensable tool for gleaning information from subcellular events within …

Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation

WR Zipfel, RM Williams, R Christie… - Proceedings of the …, 2003 - National Acad Sciences
Multicolor nonlinear microscopy of living tissue using two-and three-photon-excited intrinsic
fluorescence combined with second harmonic generation by supermolecular structures …

[HTML][HTML] Interpreting second-harmonic generation images of collagen I fibrils

RM Williams, WR Zipfel, WW Webb - Biophysical journal, 2005 - cell.com
Fibrillar collagen, being highly noncentrosymmetric, possesses a tremendous nonlinear
susceptibility. As a result, second-harmonic generation (SHG) microscopy of collagen …

The RBL-2H3 cell line: its provenance and suitability as a model for the mast cell

E Passante, N Frankish - Inflammation research, 2009 - Springer
The RBL-2H3 cell line is a commonly used histamine-releasing cell line used in
inflammation, allergy and immunological research. Quite commonly, it is referred to in …

Sequential-replenishment mechanism of exocytosis in pancreatic acini

T Nemoto, R Kimura, K Ito, A Tachikawa… - Nature cell …, 2001 - nature.com
Here we report exocytosis of zymogen granules, as examined by multiphoton excitation
imaging in intact pancreatic acini. Cholecystokinin induces Ca 2+ oscillations that trigger …

Flavoprotein autofluorescence imaging of neuronal activation in the cerebellar cortex in vivo

KC Reinert, RL Dunbar, W Gao… - Journal of …, 2004 - journals.physiology.org
Autofluorescence has been used as an indirect measure of neuronal activity in isolated cell
cultures and brain slices, but only to a limited extent in vivo. Intrinsic fluorescence signals …

[HTML][HTML] Spectrally resolved multiphoton imaging of in vivo and excised mouse skin tissues

JA Palero, HS De Bruijn, HJCM Sterenborg… - Biophysical journal, 2007 - cell.com
The deep tissue penetration and submicron spatial resolution of multiphoton microscopy
and the high detection efficiency and nanometer spectral resolution of a spectrograph were …

Fibre‐optic nonlinear optical microscopy and endoscopy

L Fu, M Gu - Journal of microscopy, 2007 - Wiley Online Library
Nonlinear optical microscopy has been an indispensable laboratory tool of high‐resolution
imaging in thick tissue and live animals. Rapid developments of fibre‐optic components in …

Multiphoton excited fluorescent materials for frequency upconversion emission and fluorescent probes

L Guo, MS Wong - Advanced materials, 2014 - Wiley Online Library
Recent progress in developing various strategies for exploiting efficient MPA fluorophores
for two emerging technological MPA applications including frequency upconversion …