SUMMARY RNA interference (RNAi) provides a powerful reverse genetics approach to
analyze gene functions both in tissue culture and in vivo. Because of its widespread …

Protein interaction networks and protein compartmentalization underlie all signaling and
regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a …

Since its introduction in 1993, the GAL4 system has become an essential part of the
Drosophila geneticist's toolkit. Widely used to drive gene expression in a multitude of cell …

To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi
Project (TRiP) at Harvard Medical School (HMS) was established along with several goals …

The impact of neonicotinoid insecticides on the health of bee pollinators is a topic of
intensive research and considerable current debate [1]. As insecticides, certain …

An expanded GGGGCC repeat in C9orf72 is the most common genetic cause of
frontotemporal dementia and amyotrophic lateral sclerosis. A fundamental question is …

Mapping neural circuits across defined synapses is essential for understanding brain
function. Here we describe trans-Tango, a technique for anterograde transsynaptic circuit …

We report a simple yet extremely efficient platform for systematic gene targeting by the RNA-
guided endonuclease Cas9 in Drosophila. The system comprises two transgenic strains …

Genetically encoded calcium indicators (GECIs) can be used to image activity in defined
neuronal populations. However, current GECIs produce inferior signals compared to …

A wide variety of biological experiments rely on the ability to express an exogenous gene in
a transgenic animal at a defined level and in a spatially and temporally controlled pattern …