Reference genes in real-time PCR
B Kozera, M Rapacz - Journal of applied genetics, 2013 - Springer
This paper aims to discuss various aspects of the use of reference genes in qPCR technique
used in the thousands of present studies. Most frequently, these are housekeeping genes …
used in the thousands of present studies. Most frequently, these are housekeeping genes …
Three important short-chain fatty acids (SCFAs) attenuate the inflammatory response induced by 5-FU and maintain the integrity of intestinal mucosal tight junction
X Yue, S Wen, D Long-Kun, Y Man, S Chang, Z Min… - BMC immunology, 2022 - Springer
Abstract Background 5-Fluorouracil (5-FU) is a used chemotherapy drug for cancer, and its
main side effect is intestinal mucositis which causes chemotherapy to fail. It was known that …
main side effect is intestinal mucositis which causes chemotherapy to fail. It was known that …
18S rRNAis a reliable normalisation gene for real time PCR based on influenza virus infected cells
SV Kuchipudi, M Tellabati, RK Nelli, GA White… - Virology journal, 2012 - Springer
Background One requisite of quantitative reverse transcription PCR (qRT-PCR) is to
normalise the data with an internal reference gene that is invariant regardless of treatment …
normalise the data with an internal reference gene that is invariant regardless of treatment …
Role of the adipose PPARγ-adiponectin axis in susceptibility to stress and depression/anxiety-related behaviors
M Guo, C Li, Y Lei, S Xu, D Zhao, XY Lu - Molecular psychiatry, 2017 - nature.com
Adaptive responses to stressful stimuli involving behavioral, emotional and metabolic
changes are orchestrated by the nervous and endocrine systems. Adipose tissue has been …
changes are orchestrated by the nervous and endocrine systems. Adipose tissue has been …
Histological evidence: housekeeping genes beta-actin and GAPDH are of limited value for normalization of gene expression
J Lin, C Redies - Development genes and evolution, 2012 - Springer
Housekeeping genes are widely used as internal controls for gene expression normalization
for western blotting, northern blotting, RT-PCR, etc. They are generally thought to be …
for western blotting, northern blotting, RT-PCR, etc. They are generally thought to be …
Inflammatory cytokine profiles in visceral and subcutaneous adipose tissues of obese patients undergoing bariatric surgery reveal lack of correlation with obesity or …
V Rakotoarivelo, G Lacraz, M Mayhue, C Brown… - …, 2018 - thelancet.com
Population studies have linked insulin resistance to systemic low-grade chronic
inflammation and have reported elevated levels of inflammatory cytokines such as TNFα, IL …
inflammation and have reported elevated levels of inflammatory cytokines such as TNFα, IL …
High-fat diet alters the expression of reference genes in male mice
X Fan, H Yao, X Liu, Q Shi, L Lv, P Li, R Wang… - Frontiers in …, 2020 - frontiersin.org
Quantitative PCR (qPCR), the most accurate and sensitive technique for quantifying mRNA
expression, and choice of appropriate reference genes for internal error controlling in qPCR …
expression, and choice of appropriate reference genes for internal error controlling in qPCR …
New insights into the role of ribonuclease P protein subunit p30 from tumor to internal reference
J Wu, S Yu, Y Wang, J Zhu, Z Zhang - Frontiers in Oncology, 2022 - frontiersin.org
Ribonuclease P protein subunit p30 (RPP30) is a highly conserved housekeeping gene that
exists in many species and tissues throughout the three life kingdoms (archaea, bacteria …
exists in many species and tissues throughout the three life kingdoms (archaea, bacteria …
Selection and validation of reference genes for quantitative gene expression studies by real-time PCR in eggplant (Solanum melongena L)
Background Analysis of gene expression patterns leads to functional understanding of
biological processes. Quantitative real-time PCR has become the most commonly used …
biological processes. Quantitative real-time PCR has become the most commonly used …
Impact of reference gene selection for target gene normalization on experimental outcome using real-time qRT-PCR in adipocytes
BS Ferguson, H Nam, RG Hopkins, RF Morrison - PloS one, 2010 - journals.plos.org
Background With the current rise in obesity-related morbidities, real-time quantitative
reverse transcription polymerase chain reaction (qRT-PCR) has become a widely used …
reverse transcription polymerase chain reaction (qRT-PCR) has become a widely used …