Legionnaires' disease (LD) is an often severe and potentially fatal form of bacterial
pneumonia caused by an extensive list of Legionella species. These ubiquitous freshwater …

To investigate whether the tumor suppressor gene PTEN affects the activity of the androgen
receptor (AR), we monitored the expression of the apoptotic gene HA-Bax (inserted in an …

Real-time PCR has become one of the most widely used methods of gene quantitation
because it has a large dynamic range, boasts tremendous sensitivity, can be highly …

The use of the polymerase chain reaction (PCR) in molecular diagnostics has increased to
the point where it is now accepted as the gold standard for detecting nucleic acids from a …

Background: High-resolution amplicon melting analysis was recently introduced as a closed-
tube method for genotyping and mutation scanning (Gundry et al. Clin Chem 2003; 49: 396 …

Use of PCR in the field of molecular diagnostics has increased to the point where it is now
accepted as the standard method for detecting nucleic acids from a number of sample and …

High-resolution melting of DNA is a simple solution for genotyping, mutation scanning and
sequence matching. The melting profile of a PCR product depends on its GC content, length …

Background: High-resolution melting of PCR amplicons with the DNA dye LCGreen™ I was
recently introduced as a homogeneous, closed-tube method of genotyping that does not …

Background: Screening for heterozygous sequence changes in PCR products, also known
as “mutation scanning”, is an important tool for genetic research and clinical applications …

Real‐time PCR is the state‐of‐the‐art technique to quantify nucleic acids for mutation
detection, genotyping and chimerism analysis. Since its development in the 1990s, many …