Analysing microbial community composition through amplicon sequencing: from sampling to hypothesis testing
LW Hugerth, AF Andersson - Frontiers in microbiology, 2017 - frontiersin.org
Microbial ecology as a scientific field is fundamentally driven by technological advance. The
past decade's revolution in DNA sequencing cost and throughput has made it possible for …
past decade's revolution in DNA sequencing cost and throughput has made it possible for …
Design and analysis of CRISPR–Cas experiments
A large and ever-expanding set of CRISPR–Cas systems now enables the rapid and flexible
manipulation of genomes in both targeted and large-scale experiments. Numerous software …
manipulation of genomes in both targeted and large-scale experiments. Numerous software …
High-coverage whole-genome sequencing of the expanded 1000 Genomes Project cohort including 602 trios
Summary The 1000 Genomes Project (1kGP) is the largest fully open resource of whole-
genome sequencing (WGS) data consented for public distribution without access or use …
genome sequencing (WGS) data consented for public distribution without access or use …
Spatiotemporal analysis of human intestinal development at single-cell resolution
D Fawkner-Corbett, A Antanaviciute, K Parikh… - Cell, 2021 - cell.com
Development of the human intestine is not well understood. Here, we link single-cell RNA
sequencing and spatial transcriptomics to characterize intestinal morphogenesis through …
sequencing and spatial transcriptomics to characterize intestinal morphogenesis through …
Local hyperthermia therapy induces browning of white fat and treats obesity
Y Li, D Wang, X Ping, Y Zhang, T Zhang, L Wang, L Jin… - Cell, 2022 - cell.com
Beige fat plays key roles in the regulation of systemic energy homeostasis; however,
detailed mechanisms and safe strategy for its activation remain elusive. In this study, we …
detailed mechanisms and safe strategy for its activation remain elusive. In this study, we …
RNA-Seq signatures normalized by mRNA abundance allow absolute deconvolution of human immune cell types
The molecular characterization of immune subsets is important for designing effective
strategies to understand and treat diseases. We characterized 29 immune cell types within …
strategies to understand and treat diseases. We characterized 29 immune cell types within …
[HTML][HTML] Global characterization of macrophage polarization mechanisms and identification of M2-type polarization inhibitors
Macrophages undergoing M1-versus M2-type polarization differ significantly in their cell
metabolism and cellular functions. Here, global quantitative time-course proteomics and …
metabolism and cellular functions. Here, global quantitative time-course proteomics and …
Allele-defined genome of the autopolyploid sugarcane Saccharum spontaneum L.
Modern sugarcanes are polyploid interspecific hybrids, combining high sugar content from
Saccharum officinarum with hardiness, disease resistance and ratooning of Saccharum …
Saccharum officinarum with hardiness, disease resistance and ratooning of Saccharum …
[HTML][HTML] Enterochromaffin cells are gut chemosensors that couple to sensory neural pathways
Dietary, microbial, and inflammatory factors modulate the gut-brain axis and influence
physiological processes ranging from metabolism to cognition. The gut epithelium is a …
physiological processes ranging from metabolism to cognition. The gut epithelium is a …
[HTML][HTML] Peripherally derived macrophages can engraft the brain independent of irradiation and maintain an identity distinct from microglia
Peripherally derived macrophages infiltrate the brain after bone marrow transplantation and
during central nervous system (CNS) inflammation. It was initially suggested that these …
during central nervous system (CNS) inflammation. It was initially suggested that these …