Yeast expression systems: overview and recent advances

R Baghban, S Farajnia, M Rajabibazl, Y Ghasemi… - Molecular …, 2019 - Springer
Yeasts are outstanding hosts for the production of functional recombinant proteins with
industrial or medical applications. Great attention has been emerged on yeast due to the …

Challenges and progress towards industrial recombinant protein production in yeasts: a review

P De Brabander, E Uitterhaegen, T Delmulle… - Biotechnology …, 2023 - Elsevier
Recombinant proteins (RP) are widely used as biopharmaceuticals, industrial enzymes, or
sustainable food source. Yeasts, with their ability to produce complex proteins through a …

[HTML][HTML] Cultivation strategies to enhance productivity of Pichia pastoris: a review

V Looser, B Bruhlmann, F Bumbak, C Stenger… - Biotechnology …, 2015 - Elsevier
Pichia pastoris, a methylotrophic yeast, is an established system for the production of
heterologous proteins, particularly biopharmaceuticals and industrial enzymes. To maximise …

Heterologous protein production using the Pichia pastoris expression system

S Macauley‐Patrick, ML Fazenda, B McNeil… - Yeast, 2005 - Wiley Online Library
The Pichia pastoris expression system is being used successfully for the production of
various recombinant heterologous proteins. Recent developments with respect to the Pichia …

Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review

O Cos, R Ramón, JL Montesinos, F Valero - Microbial cell factories, 2006 - Springer
The methylotrophic yeast Pichia pastoris has been widely reported as a suitable expression
system for heterologous protein production. The use of different phenotypes under PAOX …

Expression of Recombinant Proteins in Pichia Pastoris

P Li, A Anumanthan, XG Gao, K Ilangovan… - Applied biochemistry …, 2007 - Springer
Pichia pastoris has been used extensively and successfully to express recombinant
proteins. In this review, we summarize the elements required for expressing heterologous …

Bioprocess engineering aspects of heterologous protein production in Pichia pastoris: a review

G Potvin, A Ahmad, Z Zhang - Biochemical Engineering Journal, 2012 - Elsevier
Pichia pastoris is currently one of the most effective and versatile systems for the expression
of heterologous proteins. Its success is due to its powerful methanol-inducible alcohol …

Stress in recombinant protein producing yeasts

D Mattanovich, B Gasser, H Hohenblum… - Journal of biotechnology, 2004 - Elsevier
It is well established today that heterologous overexpression of proteins is connected with
different stress reactions. The expression of a foreign protein at a high level may either …

Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures

M Jahic, F Wallberg, M Bollok, P Garcia… - Microbial cell factories, 2003 - Springer
Background A temperature limited fed-batch (TLFB) technique is described and used for
Pichia pastoris Mut+ strain cultures and compared with the traditional methanol limited fed …

Enhancing functional production of G protein‐coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen

N André, N Cherouati, C Prual, T Steffan… - Protein …, 2006 - Wiley Online Library
We have optimized the expression level of 20 mammalian G protein‐coupled receptors
(GPCRs) in the methylotrophic yeast Pichia pastoris. We found that altering expression …