Protein phosphorylation is an essential post-translational modification that regulates many
aspects of cellular physiology, and dysregulation of pivotal phosphorylation events is often …

The extensively studied cAMP-dependent protein kinase A (PKA) is involved in the
regulation of critical cell processes, including metabolism, gene expression, and cell …

Efficient nutrient acquisition in the human gut is essential for microbial persistence. Although
polysaccharides have been well-studied nutrients for the gut microbiome, other resources …

Terpenes are the largest group of natural products with important and diverse biological
roles, while of tremendous economic value as fragrances, flavours and pharmaceutical …

A-type resistance towards “last-line” glycopeptide antibiotic vancomycin in the leading
hospital acquired infectious agent, the enterococci, is the most common in the UK …

Mammalian plasma membrane proteins make up the largest class of drug targets yet are
difficult to study in a cell free system because of their intransigent nature. Herein, we perform …

Background Increasing efforts have been made to assess the potential of Escherichia coli
strains for the production of complex recombinant proteins. Since a considerable part of …

Nucleoside analogues are a valuable experimental tool. Incorporation of these molecules
into newly synthesized DNA (ie pulse-labeling) is used to monitor cell proliferation or to …

We present a versatile method to characterize ATPase and kinase activities and discover
new inhibitors of these proteins. The proton NMR-based assay directly monitors ATP …

Background Recombinant protein-based therapeutics have become indispensable for the
treatment of many diseases. They are produced using well-established expression systems …