Investigating the conformational dynamics of a Y-family DNA polymerase during its folding and binding to DNA and a nucleotide
During DNA polymerization, the Y-family DNA polymerases are capable of bypassing
various DNA damage, which can stall the replication fork progression. It has been well …
various DNA damage, which can stall the replication fork progression. It has been well …
Kinetic investigation of the polymerase and exonuclease activities of human DNA polymerase ε holoenzyme
WJ Zahurancik, Z Suo - Journal of Biological Chemistry, 2020 - ASBMB
In eukaryotic DNA replication, DNA polymerase ε (Polε) is responsible for leading strand
synthesis, whereas DNA polymerases α and δ synthesize the lagging strand. The human …
synthesis, whereas DNA polymerases α and δ synthesize the lagging strand. The human …
Investigating the trade-off between folding and function in a multidomain Y-family DNA polymerase
The way in which multidomain proteins fold has been a puzzling question for decades. Until
now, the mechanisms and functions of domain interactions involved in multidomain protein …
now, the mechanisms and functions of domain interactions involved in multidomain protein …
Conformational Insights into the Mechanism of Acetylaminofluorene-dG-Induced Frameshift Mutations in the NarI Mutational Hotspot
L Xu, BP Cho - Chemical research in toxicology, 2016 - ACS Publications
Frameshift mutagenesis encompasses the gain or loss of DNA base pairs, resulting in
altered genetic outcomes. The Nar I restriction site sequence 5′-G1G2CG3C X-3′ in …
altered genetic outcomes. The Nar I restriction site sequence 5′-G1G2CG3C X-3′ in …
Pre-steady-state kinetic investigation of bypass of a bulky guanine lesion by human Y-family DNA polymerases
EJ Tokarsky, VV Gadkari, WJ Zahurancik, CK Malik… - DNA repair, 2016 - Elsevier
Nitrobenzanthrone (3-NBA), a byproduct of diesel exhaust, is highly present in the
environment and poses a significant health risk. Exposure to 3-NBA results in formation of N …
environment and poses a significant health risk. Exposure to 3-NBA results in formation of N …
DNA Polymerase ν Rapidly Bypasses O6-Methyl-dG but Not O6-[4-(3-Pyridyl)-4-oxobutyl-dG and O2-Alkyl-dTs
ASP Gowda, TE Spratt - Chemical research in toxicology, 2016 - ACS Publications
4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a potent tobacco carcinogen that
forms mutagenic DNA adducts including O 6-methyl-2′-deoxyguanosine (O 6-Me-dG), O 6 …
forms mutagenic DNA adducts including O 6-methyl-2′-deoxyguanosine (O 6-Me-dG), O 6 …
Backbone assignment of the binary complex of the full length Sulfolobus solfataricus DNA polymerase IV and DNA
Sulfolobus solfataricus DNA polymerase IV (Dpo4), a model Y-family DNA polymerase,
bypasses a wide range of DNA lesions in vitro and in vivo. In this paper, we report the …
bypasses a wide range of DNA lesions in vitro and in vivo. In this paper, we report the …
Advances in Structural and Single-Molecule Methods for Investigating DNA Lesion Bypass and Repair Polymerases
Innovative advances in X-ray crystallography and single-molecule biophysics have yielded
unprecedented insight into the mechanisms of DNA lesion bypass and damage repair. Time …
unprecedented insight into the mechanisms of DNA lesion bypass and damage repair. Time …
Increased processivity, misincorporation, and nucleotide incorporation efficiency in Sulfolobus solfataricus Dpo4 thumb domain mutants
L Wang, C Liang, J Wu, L Liu… - Applied and Environmental …, 2017 - Am Soc Microbiol
The present study aimed to increase the processivity of Sulfolobus solfataricus DNA
polymerase Dpo4. Protein engineering and bioinformatics were used to compile a library of …
polymerase Dpo4. Protein engineering and bioinformatics were used to compile a library of …
Translesion synthesis past guanine (C8)–thymine (N3) intrastrand cross-links catalyzed by selected A-and Y-family polymerases
YA Lee, YC Lee, NE Geacintov, V Shafirovich - Molecular BioSystems, 2016 - pubs.rsc.org
Oxidatively generated guanine radicals in DNA can undergo various nucleophilic reactions
including the formation of C8-guanine cross-links with adjacent or nearby N3-thymines in …
including the formation of C8-guanine cross-links with adjacent or nearby N3-thymines in …