Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

A Stolz, K Jooß, O Höcker, J Römer, J Schlecht… - …, 2019 - Wiley Online Library
Capillary electrophoresis (CE) offers fast and high‐resolution separation of charged
analytes from small injection volumes. Coupled to mass spectrometry (MS), it represents a …

Microfluidics add-on technologies for single-cell analysis

M Khan, Y Bi, G Zhang, F Yin, Y Xie, L Lin… - TrAC Trends in Analytical …, 2023 - Elsevier
Microfluidics revolutionized single-cell analysis in a way that was impossible through
conventional methods. However, microfluidic is not a stand-alone technique and cannot …

Deciphering metabolic heterogeneity by single-cell analysis

TMJ Evers, M Hochane, SJ Tans, RMA Heeren… - 2019 - ACS Publications
Single-cell analysis provides insights into cellular heterogeneity and dynamics of individual
cells. This Feature highlights recent developments in key analytical techniques suited for …

Patch-clamp proteomics of single neurons in tissue using electrophysiology and subcellular capillary electrophoresis mass spectrometry

SB Choi, AM Polter, P Nemes - Analytical chemistry, 2021 - ACS Publications
Understanding of the relationship between cellular function and molecular composition
holds a key to next-generation therapeutics but requires measurement of all types of …

Capillary electrophoresis mass spectrometry for scalable single-cell proteomics

B Shen, LR Pade, SB Choi, P Muñoz-LLancao… - Frontiers in …, 2022 - frontiersin.org
Understanding the biochemistry of the cell requires measurement of all the molecules it
produces. Single-cell proteomics recently became possible through advances in …

Data-dependent acquisition ladder for capillary electrophoresis mass spectrometry-based ultrasensitive (neuro) proteomics

SB Choi, P Muñoz-LLancao, MC Manzini… - Analytical …, 2021 - ACS Publications
Measurement of broad types of proteins from a small number of cells to single cells would
help to better understand the nervous system but requires significant leaps in sensitivity in …

Microanalysis of brain angiotensin peptides using ultrasensitive capillary electrophoresis trapped ion mobility mass spectrometry

K DeLaney, D Jia, L Iyer, Z Yu, SB Choi… - Analytical …, 2022 - ACS Publications
While the role of the renin–angiotensin system (RAS) in peripheral circulation is well
characterized, we still lack an in-depth understanding of its role within the brain. This …

Capillary zone electrophoresis-mass spectrometry for bottom-up proteomics

Z Zhang, Y Qu, NJ Dovichi - TrAC Trends in Analytical Chemistry, 2018 - Elsevier
Bottom-up proteomics characterizes proteins by analysis of peptides generated through
proteolysis. Bottom-up analysis of a complex proteome inevitably generates tens of …

Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (2015–mid 2018)

S Štěpánová, V Kašička - Journal of separation science, 2019 - Wiley Online Library
This review summarizes recent developments and applications of capillary and microchip
electroseparation methods in proteomic and peptidomic analyses since the year 2015 to ca …

Recent advances and new perspectives in capillary electrophoresis-mass spectrometry for single cell “omics”

K DeLaney, CS Sauer, NQ Vu, L Li - Molecules, 2018 - mdpi.com
Accurate clinical therapeutics rely on understanding the metabolic responses of individual
cells. However, the high level of heterogeneity between cells means that simply sampling …