Cryopreservation of semen in domestic animals: A review of current challenges, applications, and prospective strategies

M Sharafi, SM Borghei-Rad, M Hezavehei, A Shahverdi… - Animals, 2022 - mdpi.com
Simple Summary Here we present a comprehensive review of the current literature
describing mechanisms of damage incurred by cryopreservation, and we highlight several …

Antioxidants and their effect on the oxidative/nitrosative stress of frozen-thawed boar sperm

F Pezo, M Yeste, F Zambrano, P Uribe, J Risopatrón… - Cryobiology, 2021 - Elsevier
In swine, the use of frozen-thawed boar sperm for artificial insemination remains a
suboptimal reproductive technology. Among the negative effects of cryopreservation on …

Cryopreservation induces acetylation of metabolism-related proteins in boar sperm

MA Ali, Z Qin, S Dou, A Huang, Y Wang… - International Journal of …, 2023 - mdpi.com
Cryodamage affects the normal physiological functions and survivability of boar sperm
during cryopreservation. Lysine acetylation is thought to be an important regulatory …

[HTML][HTML] Spermatozoa and seminal plasma small extracellular vesicles miRNAs as biomarkers of boar semen cryotolerance

AC Pedrosa, MA Torres, DV Alkmin, JEP Pinzon… - Theriogenology, 2021 - Elsevier
Freeze boar semen is still the biggest challenge for the swine industry due to the high cold
shock sensitivity of boar sperm cells and the variance of post-thaw results among individuals …

In stallion spermatozoa, superoxide dismutase (cu–Zn)(SOD1) and the Aldo-keto-reductase family 1 member b (AKR1B1) are the proteins most significantly reduced …

G Gaitskell-Phillips, FE Martín-Cano… - Journal of Proteome …, 2021 - ACS Publications
Although cryopreservation is widely used in animal breeding, the technique is still
suboptimal. The population of spermatozoa surviving the procedure experiences changes …

Differences in the proteome of stallion spermatozoa explain stallion-to-stallion variability in sperm quality post-thaw

G Gaitskell-Phillips, FE Martín-Cano… - Biology of …, 2021 - academic.oup.com
The identification of stallions and or ejaculates that will provide commercially acceptable
quality post-thaw before cryopreservation is of great interest, avoiding wasting time and …

The role of seminal plasma in the liquid storage of spermatozoa

L Höfner, AM Luther, D Waberski - Animal reproduction science, 2020 - Elsevier
Ongoing progress in proteomic characterization of seminal plasma has stimulated research
on the identification of biomarkers for male fertility and sperm preservability. So far, many …

Direct but not indirect methods correlate the percentages of sperm with altered chromatin to the intensity of chromatin damage

J Ribas-Maynou, M Llavanera… - Frontiers in Veterinary …, 2021 - frontiersin.org
Although sperm chromatin damage, understood as damage to DNA or affectations in sperm
protamination, has been proposed as a biomarker for sperm quality in both humans and …

Proteomics in fresh and preserved pig semen: Recent achievements and future challenges

J Roca, C Perez-Patino, I Barranco, LC Padilla… - Theriogenology, 2020 - Elsevier
Proteins in semen, either in spermatozoa (SPZ) or seminal plasma (SP), are directly
involved in molecular processes and biological pathways regulating sperm function …

Comparative iTRAQ proteomics identified proteins in fresh and frozen thawed yak spermatozoa

W Qin, M Chu, X Ma, J Pei, L Xiong, P Bao, Y La… - International Journal of …, 2023 - Elsevier
The changes in semen and cryodamage after the cryopreservation process negatively affect
sperm function and motility. However, possible proteomic alterations of yak semen during …