Bacillus subtilis is the most characterized gram-positive bacterium that has significant
attributes, such as growing well on cheap carbon sources, possessing clear inherited …

Bacillus subtilis is a highly promising production system for various biomolecules. This
review begins with the algorithm of fed-batch operations (FBOs) and then illustrates the …

Dynamic regulation is a promising strategy for fine-tuning metabolic fluxes in microbial cell
factories. However, few of these synthetic regulatory systems have been developed for …

Genetic elements are key components of metabolic engineering and synthetic biological
applications, allowing the development of organisms as biosensors and for manufacturing …

Bacillus subtilis as an important host has been widely used in synthetic biology, metabolic
engineering, and production of industrial enzymes. To fully take advantage of this organism …

Background Engineering bacteria with the purpose of optimizing the production of
interesting molecules often leads to a decrease in growth due to metabolic burden or …

Gene expression in spatiotemporal distribution improves the ability of cells to respond to
changing environments. For microbial cell factories in artificial environments, reconstruction …

Improving the production of lignocellulose-degrading enzyme such as cellulase and
xylanase substantially increases the chance for cost-competitive production of cellulosic …

Inducer-free integrative vectors are often used to create B. subtilis strains for industrial
purposes, but employing strong promoters to produce high levels of recombinant proteins in …

In the present study, the production of cellulase by Trichoderma reesei under solid-state
fermentation of nettle biomass was promoted through supplementation of the culture media …