Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

Cryo-electron tomography and subtomogram averaging (STA) has developed rapidly in
recent years. It provides structures of macromolecular complexes in situ and in cellular …

We present a new approach for macromolecular structure determination from multiple
particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram …

Single-particle cryo-electron tomography is an emerging technique capable of determining
the structure of proteins imaged within the native context of cells at molecular resolution …

Summary Approximately 30%–40% of global CO 2 fixation occurs inside a non-membrane-
bound organelle called the pyrenoid, which is found within the chloroplasts of most …

Cryo-electron microscopy (cryo-EM) has emerged as a powerful structure determination
technique. Its most prolific branch is single particle analysis (SPA), a method being used in a …

Macromolecular complexes are intrinsically flexible and often challenging to purify for
structure determination by single-particle cryo-electron microscopy (cryo-EM). Such …

Cryo-electron tomography (cryo-ET) allows 3D volumes to be reconstructed from a set of 2D
projection images of a tilted biological sample. It allows densities to be resolved in 3D that …

Protein synthesis, transport, and N-glycosylation are coupled at the mammalian
endoplasmic reticulum by complex formation of a ribosome, the Sec61 protein-conducting …

Electron microscopy played a key role in establishing cell biology as a discipline, by
producing fundamental insights into cellular organization and ultrastructure. Many seminal …