Native mass spectrometry (MS) involves the analysis and characterization of
macromolecules, predominantly intact proteins and protein complexes, whereby as much as …

The rise of the “Top Down” method in the field of mass spectrometry-based proteomics has
ushered in a new age of promise and challenge for the characterization and identification of …

Mass spectrometry is unmatched in its versatility for studying practically any aspect of the
proteome. Because the foundations of mass spectrometry-based proteomics are complex …

Interpretation of mass spectra is challenging because they report a ratio of two physical
quantities, mass and charge, which may each have multiple components that overlap in m/z …

Deisotoping, or the process of removing peaks in a mass spectrum resulting from the
incorporation of naturally occurring heavy isotopes, has long been used to reduce …

A proteoform is a defined form of a protein derived from a given gene with a specific amino
acid sequence and localized post‐translational modifications. In top‐down proteomic …

A full description of the human proteome relies on the challenging task of detecting mature
and changing forms of protein molecules in the body. Large-scale proteome analysis has …

Background Mass spectrometry is an essential analytical technique for high-throughput
analysis in proteomics and metabolomics. The development of new separation techniques …

Extraction of data from the proprietary RAW files generated by Thermo Fisher mass
spectrometers is the primary step for subsequent data analysis. High resolution and high …

Beyond the genetic code, the human proteome is greatly diversified by genetic variations,
alternative splicing of RNA transcripts, and post-translational modifications (PTMs). 6, 7 In …