Applications of bioluminescence in biotechnology and beyond
AJ Syed, JC Anderson - Chemical Society Reviews, 2021 - pubs.rsc.org
Bioluminescence is the fascinating natural phenomenon by which living creatures produce
light. Bioluminescence occurs when the oxidation of a small-molecule luciferin is catalysed …
light. Bioluminescence occurs when the oxidation of a small-molecule luciferin is catalysed …
NanoBRET: the bright future of proximity-based assays
NC Dale, EKM Johnstone, CW White… - … in bioengineering and …, 2019 - frontiersin.org
Bioluminescence resonance energy transfer (BRET) is a biophysical technique used to
monitor proximity within live cells. BRET exploits the naturally occurring phenomenon of …
monitor proximity within live cells. BRET exploits the naturally occurring phenomenon of …
Fluorescent ligands: Bringing light to emerging GPCR paradigms
In recent years, several novel aspects of GPCR pharmacology have been described, which
are thought to play a role in determining the in vivo efficacy of a compound. Fluorescent …
are thought to play a role in determining the in vivo efficacy of a compound. Fluorescent …
Development of fluorescent AF64394 analogues enables real-time binding studies for the orphan class A GPCR GPR3
The orphan G protein-coupled receptor (oGPCR) GPR3 represents a potential drug target
for the treatment of Alzheimer's disease and metabolic disorders. However, the limited …
for the treatment of Alzheimer's disease and metabolic disorders. However, the limited …
Pulmonary myeloid cell uptake of biodegradable nanoparticles conjugated with an anti-fibrotic agent provides a novel strategy for treating chronic allergic airways …
Asthma (chronic allergic airways disease, AAD) is characterized by airway inflammation (AI),
airway remodeling (AWR) and airway hyperresponsiveness (AHR). Current treatments for …
airway remodeling (AWR) and airway hyperresponsiveness (AHR). Current treatments for …
The relaxin family peptide receptor 1 (RXFP1): An emerging player in human health and disease
TY Chen, X Li, CH Hung… - … Genetics & Genomic …, 2020 - Wiley Online Library
Background Relaxin/relaxin family peptide receptor 1 (RXFP1) signaling is important for
both normal physiology and disease. Strong preclinical evidence supports relaxin as a …
both normal physiology and disease. Strong preclinical evidence supports relaxin as a …
NanoBRET binding assay for histamine H2 receptor ligands using live recombinant HEK293T cells
L Grätz, K Tropmann, M Bresinsky, C Müller… - Scientific Reports, 2020 - nature.com
Fluorescence/luminescence-based techniques play an increasingly important role in the
development of test systems for the characterization of future drug candidates, especially in …
development of test systems for the characterization of future drug candidates, especially in …
Development of a synthetic relaxin-3/INSL5 chimeric peptide ligand for NanoBiT complementation binding assays
H Wu, BL Hoare, TNG Handley, MA Hossain… - Biochemical …, 2024 - Elsevier
INSL5 and relaxin-3 are relaxin family peptides with important roles in gut and brain
function, respectively. They mediate their actions through the class A GPCRs RXFP4 and …
function, respectively. They mediate their actions through the class A GPCRs RXFP4 and …
[HTML][HTML] Red-shifted bioluminescence Resonance Energy Transfer: Improved tools and materials for analytical in vivo approaches
Abstract Bioluminescence Resonance Energy Transfer (BRET) has been proven as a highly
sensitive optical transduction system for bio-imaging and a variety of analytical approaches …
sensitive optical transduction system for bio-imaging and a variety of analytical approaches …
Functional crosstalk between angiotensin receptors (types 1 and 2) and relaxin family peptide receptor 1 (RXFP1): Implications for the therapeutic targeting of fibrosis
Class A, rhodopsin‐like, G‐protein‐coupled receptors (GPCRs) are by far the largest class
of GPCRs and are integral membrane proteins used by various cells to convert extracellular …
of GPCRs and are integral membrane proteins used by various cells to convert extracellular …