Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

M Ahmad, M Hirz, H Pichler, H Schwab - Applied microbiology and …, 2014 - Springer
Pichia pastoris is an established protein expression host mainly applied for the production of
biopharmaceuticals and industrial enzymes. This methylotrophic yeast is a distinguished …

[HTML][HTML] Metabolic engineering of Pichia pastoris

DA Peña, B Gasser, J Zanghellini, MG Steiger… - Metabolic …, 2018 - Elsevier
Besides its use for efficient production of recombinant proteins the methylotrophic yeast
Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to …

Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris

P Cai, X Duan, X Wu, L Gao, M Ye… - Nucleic Acids …, 2021 - academic.oup.com
The industrial yeast Pichia pastoris has been harnessed extensively for production of
proteins, and it is attracting attention as a chassis cell factory for production of chemicals …

Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design

X Lu, Y Liu, Y Yang, S Wang, Q Wang, X Wang… - Nature …, 2019 - nature.com
Acetyl-CoA is a fundamental metabolite for all life on Earth, and is also a key starting point
for the biosynthesis of a variety of industrial chemicals and natural products. Here we design …

Genome sequence of the model medicinal mushroom Ganoderma lucidum

S Chen, J Xu, C Liu, Y Zhu, DR Nelson, S Zhou… - Nature …, 2012 - nature.com
Ganoderma lucidum is a widely used medicinal macrofungus in traditional Chinese
medicine that creates a diverse set of bioactive compounds. Here we report its 43.3-Mb …

Improving industrial yeast strains: exploiting natural and artificial diversity

J Steensels, T Snoek, E Meersman… - FEMS microbiology …, 2014 - academic.oup.com
Yeasts have been used for thousands of years to make fermented foods and beverages,
such as beer, wine, sake, and bread. However, the choice for a particular yeast strain or …

Heterologous Protein Expression in Pichia pastoris: Latest Research Progress and Applications

V Juturu, JC Wu - ChemBioChem, 2018 - Wiley Online Library
Pichia pastoris is a well‐known platform strain for heterologous protein expression. Over the
past five years, different strategies to improve the efficiency of recombinant protein …

Production of recombinant proteins by yeast cells

E Çelik, P Çalık - Biotechnology advances, 2012 - Elsevier
Yeasts are widely used in production of recombinant proteins of medical or industrial
interest. For each individual product, the most suitable expression system has to be …

Recombinant protein production in yeasts

D Mattanovich, P Branduardi, L Dato, B Gasser… - Recombinant gene …, 2012 - Springer
Recombinant protein production is a multibillion-dollar market. The development of a new
product begins with the choice of a production host. While one single perfect host for every …

Overcoming barriers to membrane protein structure determination

RM Bill, PJF Henderson, S Iwata, ERS Kunji… - Nature …, 2011 - nature.com
After decades of slow progress, the pace of research on membrane protein structures is
beginning to quicken thanks to various improvements in technology, including protein …