Differential RNA-seq: the approach behind and the biological insight gained

CM Sharma, J Vogel - Current opinion in microbiology, 2014 - Elsevier
Highlights•RNA-seq has become the method of choice for bacterial transcriptomics.•
Differential RNA-seq (dRNA-seq) distinguishes primary and processed transcripts.•dRNA …

Global transcriptional start site mapping using differential RNA sequencing reveals novel antisense RNAs in Escherichia coli

MK Thomason, T Bischler, SK Eisenbart… - Journal of …, 2015 - Am Soc Microbiol
While the model organism Escherichia coli has been the subject of intense study for
decades, the full complement of its RNAs is only now being examined. Here we describe a …

CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains

PR Wright, J Georg, M Mann, DA Sorescu… - Nucleic acids …, 2014 - academic.oup.com
CopraRNA (Comparative prediction algorithm for small RNA targets) is the most recent asset
to the Freiburg RNA Tools webserver. It incorporates and extends the functionality of the …

Identification of NAD-RNA species and ADPR-RNA decapping in Archaea

JV Gomes-Filho, R Breuer, HG Morales-Filloy… - Nature …, 2023 - nature.com
NAD is a coenzyme central to metabolism that also serves as a 5′-terminal cap for bacterial
and eukaryotic transcripts. Thermal degradation of NAD can generate nicotinamide and …

tappAS: a comprehensive computational framework for the analysis of the functional impact of differential splicing

L de la Fuente, Á Arzalluz-Luque, M Tardáguila… - Genome Biology, 2020 - Springer
Recent advances in long-read sequencing solve inaccuracies in alternative transcript
identification of full-length transcripts in short-read RNA-Seq data, which encourages the …

Long-read sequencing of human cytomegalovirus transcriptome reveals RNA isoforms carrying distinct coding potentials

Z Balázs, D Tombácz, A Szűcs, Z Csabai, K Megyeri… - Scientific reports, 2017 - nature.com
The human cytomegalovirus (HCMV) is a ubiquitous, human pathogenic herpesvirus. The
complete viral genome is transcriptionally active during infection; however, a large part of its …

ReadXplorer 2—detailed read mapping analysis and visualization from one single source

R Hilker, KB Stadermann, O Schwengers… - …, 2016 - academic.oup.com
Motivation: The vast amount of already available and currently generated read mapping
data requires comprehensive visualization, and should benefit from bioinformatics tools …

Genome-Wide Transcription Start Site Mapping and Promoter Assignments to a Sigma Factor in the Human Enteropathogen Clostridioides difficile

O Soutourina, T Dubois, M Monot… - Frontiers in …, 2020 - frontiersin.org
The emerging human enteropathogen Clostridioides difficile is the main cause of diarrhea
associated with antibiotherapy. Regulatory pathways underlying the adaptive responses …

ANNOgesic: a Swiss army knife for the RNA-seq based annotation of bacterial/archaeal genomes

SH Yu, J Vogel, KU Förstner - Gigascience, 2018 - academic.oup.com
To understand the gene regulation of an organism of interest, a comprehensive genome
annotation is essential. While some features, such as coding sequences, can be …

Third-generation sequencing reveals extensive polycistronism and transcriptional overlapping in a baculovirus

N Moldován, D Tombácz, A Szűcs, Z Csabai… - Scientific Reports, 2018 - nature.com
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an insect-pathogen
baculovirus. In this study, we applied the Oxford Nanopore Technologies platform for the …