Multiplex staining by sequential immunostaining and antibody removal on routine tissue sections

MM Bolognesi, M Manzoni, CR Scalia… - … of Histochemistry & …, 2017 - journals.sagepub.com
Multiplexing, labeling for multiple immunostains in the very same cell or tissue section in
situ, has raised considerable interest. The methods proposed include the use of labeled …

Antigen masking during fixation and embedding, dissected

CR Scalia, G Boi, MM Bolognesi… - … of Histochemistry & …, 2017 - journals.sagepub.com
Antigen masking in routinely processed tissue is a poorly understood process caused by
multiple factors. We sought to dissect the effect on antigenicity of each step of processing by …

[HTML][HTML] Accelerated instability testing reveals quantitative mass spectrometry overcomes specimen storage limitations associated with PD-L1 immunohistochemistry

A Haragan, DC Liebler, DM Das, MD Soper… - Laboratory …, 2020 - Elsevier
Immunohistochemistry (IHC) using formalin-fixed, paraffin embedded (FFPE) tissue is limited
by epitope masking, posttranslational modification and immunoreactivity loss that occurs in …

Mechanistic considerations for adenosine-lidocaine-magnesium (ALM) in controlling coagulopathy

E Biros, E Birosova, CS Moran - Trends in Pharmacological Sciences, 2023 - cell.com
Abstract Adenosine-lidocaine-magnesium (ALM) mixture is a cardioplegic agent that
improves survivability undisputedly in rodents, but not swine, models of hemorrhagic shock …

Modulation of the cardiac myocyte action potential by the magnesium-sensitive TRPM6 and TRPM7-like current

A Gwanyanya, I Andriulė, BM Istrate, F Easmin… - International Journal of …, 2021 - mdpi.com
The cardiac Mg2+-sensitive, TRPM6, and TRPM7-like channels remain undefined,
especially with the uncertainty regarding TRPM6 expression in cardiomyocytes. Additionally …

Optic nerve injury upregulates retinoic acid signaling in the adult frog visual system

MV Duprey-Díaz, JM Blagburn, RE Blanco - Journal of chemical …, 2016 - Elsevier
Retinoic acid (RA) is important during development, in neuronal plasticity, and also in
peripheral nervous system regeneration. Here we use the frog visual system as a model to …

Antibodies validated for routinely processed tissues stain frozen sections unpredictably

MM Bolognesi, F Mascadri, L Furia, M Faretta… - …, 2021 - Taylor & Francis
Background: Antibody validation for tissue staining is required for reproducibility; criteria to
ensure validity have been published recently. The majority of these recommendations imply …

Indirect Methods to Determine the Risk of Damage to the Health of Firefighters and Children Due to Exposure to Smoke Emission from Burning Wood/Coal in a …

MS Ocampos, LCS Leite, ES de Pádua Melo… - International Journal of …, 2023 - mdpi.com
People are constantly exposed to particulate matter and chemicals released during fires.
However, there are still few studies on gas and particulate emissions related to exposure to …

TdT expression in germ cell tumours: a possible immunohistochemical cross-reaction and diagnostic pitfall

M Jaconi, F Magni, F Raimondo, M Ponzoni… - Journal of Clinical …, 2019 - jcp.bmj.com
Aims Very recent papers proposed a possible role for the expression of terminal
deoxynucleotidyl transferase (TdT) in the tumourigenesis of gonadal and extragonadal germ …

Synthetic antigen gels as practical controls for standardized and quantitative immunohistochemistry

KJ Hötzel, CA Havnar, HV Ngu, S Rost… - … of Histochemistry & …, 2019 - journals.sagepub.com
Optimization and standardization of immunohistochemistry (IHC) protocols within and
between laboratories requires reproducible positive and negative control samples. In many …