Real-time PCR is the method of choice in many laboratories for diagnostic and food
applications. This technology merges the polymerase chain reaction chemistry with the use …

Environmental DNA (eDNA) surveillance holds great promise for improving species
conservation and management. However, few studies have investigated eDNA dynamics …

Primers are critical components of any PCR assay, as they are the main determinants of its
specificity, sensitivity, and robustness. Despite the publication of numerous guidelines, the …

Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse
transcribed from mRNA is on the way to becoming a routine tool in molecular biology to …

Optimization of primer sequences for polymerase chain reaction (PCR) and quantitative
PCR (qPCR) and reaction conditions remains an experimental challenge. We have …

MicroRNAs (MiRNAs) play multiple crucial regulating roles in cell which can regulate one
third of protein-coding genes. MiRNAs participate in the developmental and physiological …

DNA amplification is essential to most nucleic acid testing strategies, but established
techniques require sophisticated equipment or complex experimental procedures, and their …

To investigate whether the tumor suppressor gene PTEN affects the activity of the androgen
receptor (AR), we monitored the expression of the apoptotic gene HA-Bax (inserted in an …

Real-time PCR has become one of the most widely used methods of gene quantitation
because it has a large dynamic range, boasts tremendous sensitivity, can be highly …

ABSTRACT PrimerBank (http://pga. mgh. harvard. edu/primerbank/) is a public resource for
the retrieval of human and mouse primer pairs for gene expression analysis by PCR and …